ArticleApplied and environmental microbiology2005
Sequence and expression analyses of Cytophaga-like hydrolases in a Western arctic metagenomic library and the Sargasso Sea.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 65 citations in OpenAlex.
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases.International journal of molecular sciences · 2022Review
- The Effect of Increased Loads of Dissolved Organic Matter on Estuarine Microbial Community Composition and Function.Frontiers in microbiology · 2017Article
- Proteorhodopsin light-enhanced growth linked to vitamin-B1 acquisition in marine Flavobacteria.The ISME journal · 2016Article
- Integrated (Meta) Genomic and Synthetic Biology Approaches to Develop New Biocatalysts.Marine drugs · 2016Review
- Microbial Surface Colonization and Biofilm Development in Marine Environments.Microbiology and molecular biology reviews : MMBR · 2016Review
- Emerging strategies and integrated systems microbiology technologies for biodiscovery of marine bioactive compounds.Marine drugs · 2014Review
- Article
- Patterns of extracellular enzyme activities and microbial metabolism in an Arctic fjord of Svalbard and in the northern Gulf of Mexico: contrasts in carbon processing by pelagic microbial communities.Frontiers in microbiology · 2013Article
- Functional characterization of two M42 aminopeptidases erroneously annotated as cellulases.PloS one · 2012Article
- Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum.Journal of industrial microbiology & biotechnology · 2011Article
- Prospecting metagenomic enzyme subfamily genes for DNA family shuffling by a novel PCR-based approach.The Journal of biological chemistry · 2010Article
- Article
- The complete genome of Zunongwangia profunda SM-A87 reveals its adaptation to the deep-sea environment and ecological role in sedimentary organic nitrogen degradation.BMC genomics · 2010Article
- The biodiversity and ecology of Antarctic lakes: models for evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence analysis of environmental DNA promises to provide new insights into the ecology and biogeochemistry of uncultured marine microbes. In this study we used the Sargasso Sea Whole Genome Sequence (WGS) data set to search for hydrolases used by Cytophaga-like bacteria to degrade biopolymers such as polysaccharides and proteins. Analysis of the Sargasso WGS data for contigs bearing both the 16S rRNA genes of Cytophaga-like bacteria and hydrolase genes revealed a cellulase gene (celM) most similar to the gene found in Cytophaga hutchinsonii. A BLAST search of the entire Sargasso Sea WGS data set indicated that celM was the most abundant cellulase-like gene in the Sargasso Sea. However, the similarity between CelM-like cellulases and peptidases belonging to metalloprotease family M42 led us to question whether CelM is involved in the degradation of polysaccharides or proteins. PCR primers were designed for the celM genes in the Sargasso Sea WGS data set and used to identify celM in a fosmid library constructed with prokaryotic DNA from the western Arctic Ocean. Expression analysis of the Cytophaga-like Arctic CelM, which is 63% identical and 77% similar to CelM in C. hutchinsonii, indicated that there was peptidase activity, whereas cellulase activity was not detected. Our analysis suggests that the celM gene plays a role in the degradation of protein by Cytophaga-like bacteria. The abundance of peptidase genes in the Cytophaga-like fosmid clone provides further evidence for the importance of Cytophaga-like bacteria in the degradation of protein in high-molecular-weight dissolved organic matter.
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