ArticleApplied and environmental microbiology2005
Detection of genetic markers of fecal indicator bacteria in Lake Michigan and determination of their relationship to Escherichia coli densities using standard microbiological methods.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Comprehensive evaluation of UV inactivation ofWater research X · 2025Article
- Distributions of Fecal Indicators at Aquaculture Areas in a Bay of Republic of Korea.Journal of microbiology and biotechnology · 2024Article
- Isolation and molecular characterization of multidrug‑resistant3 Biotech · 2024Article
- Temporal patterns of bacterial communities in the Billings Reservoir system.Scientific reports · 2024Article
- Fecal indicators and antibiotic resistance genes exhibit diurnal trends in the Chattahoochee River: Implications for water quality monitoring.Frontiers in microbiology · 2022Article
- Increased Antimicrobial and Multidrug Resistance Downstream of Wastewater Treatment Plants in an Urban Watershed.Frontiers in microbiology · 2021Article
- Male-Specific and Somatic Coliphage Profiles from Major Aquaculture Areas in Republic of Korea.Food and environmental virology · 2020Article
- Ecological and Technical Mechanisms for Cross-Reaction of Human Fecal Indicators with Animal Hosts.Applied and environmental microbiology · 2020Article
- Article
- Use of coliphages to investigate norovirus contamination in a shellfish growing area in Republic of Korea.Environmental science and pollution research international · 2018Article
- Bacteroides sedimenti sp. nov., isolated from a chloroethenes-dechlorinating consortium enriched from river sediment.Journal of microbiology (Seoul, Korea) · 2018Article
- PCR data and comparative performance ofData in brief · 2018Article
- Sewage loading and microbial risk in urban waters of the Great Lakes.Elementa (Washington, D.C.) · 2018Article
- The use of wastewater in livestock production and its socioeconomic and welfare implications.Environmental science and pollution research international · 2017Review
- Assessment of bacterial and archaeal community structure in Swine wastewater treatment processes.Microbial ecology · 2015Article
- Human health-related ecosystem services of avian-dense coastal wetlands adjacent to a Western Lake Erie swimming beach.EcoHealth · 2015Article
- Distribution and abundance of human-specific Bacteroides and relation to traditional indicators in an urban tropical catchment.Journal of applied microbiology · 2014Article
- Occurrence of waterborne pathogens and Escherichia coli at offshore drinking water intakes in lake Ontario.Applied and environmental microbiology · 2013Article
- A microbial signature approach to identify fecal pollution in the waters off an urbanized coast of Lake Michigan.Microbial ecology · 2013Article
- Unsuitability of quantitative Bacteroidales 16S rRNA gene assays for discerning fecal contamination of drinking water.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lake Michigan surface waters impacted by fecal pollution were assessed to determine the occurrence of genetic markers for Bacteroides and Escherichia coli. Initial experiments with sewage treatment plant influent demonstrated that total Bacteroides spp. could be detected by PCR in a 25- to 125-fold-higher dilution series than E. coli and human-specific Bacteroides spp., which were both found in similar dilution ranges. The limit of detection for the human-specific genetic marker ranged from 0.2 CFU/100 ml to 82 CFU/100 ml culturable E. coli for four wastewater treatment plants in urban and rural areas. The spatial and temporal distributions of these markers were assessed following major rain events that introduced urban storm water, agricultural runoff, and sewage overflows into Lake Michigan. Bacteroides spp. were detected in all of these samples by PCR, including those with <1 CFU/100 ml E. coli. Human-specific Bacteroides spp. were detected as far as 2 km into Lake Michigan during sewage overflow events, with variable detection 1 to 9 days postoverflow, whereas the cow-specific Bacteroides spp. were detected in only highly contaminated samples near the river outflow. Lake Michigan beaches were also assessed throughout the summer season for the same markers. Bacteroides spp. were detected in all beach samples, including 28 of the 74 samples that did not exceed 235 CFU/100 ml of E. coli. Human-specific Bacteroides spp. were detected at three of the seven beaches; one of the sites demonstrating positive results was sampled during a reported sewage overflow, but E. coli levels were below 235 CFU/100 ml. This study demonstrates the usefulness of non-culture-based microbial-source tracking approaches and the prevalence of these genetic markers in the Great Lakes, including freshwater coastal beaches.
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