ArticleApplied and environmental microbiology2005
Protamylasse, a residual compound of industrial starch production, provides a suitable medium for large-scale cyanophycin production.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Biosynthesis of cyanophycin by high-cell-density cultivation of recombinant Escherichia coli for corrosion-resistant biomaterial applications.Bioprocess and biosystems engineering · 2026Article
- Sustainable Production of the Cyanophycin Biopolymer in Tobacco in the Greenhouse and Field.Frontiers in bioengineering and biotechnology · 2022Article
- Identification of emulsifier potato peptides by bioinformatics: application to omega-3 delivery emulsions and release from potato industry side streams.Scientific reports · 2020Article
- Guanidination of soluble lysine-rich cyanophycin yields a homoarginine-containing polyamide.Applied and environmental microbiology · 2014Article
- Increased lysine content is the main characteristic of the soluble form of the polyamide cyanophycin synthesized by recombinant Escherichia coli.Applied and environmental microbiology · 2013Article
- Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1.AMB Express · 2011Article
- Biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization.Applied and environmental microbiology · 2009Article
- Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and role of three other coisolates in a mixed bacterial consortium.Applied and environmental microbiology · 2008Article
- Assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid production.Applied microbiology and biotechnology · 2007Review
- Biomass in the manufacture of industrial products--the use of proteins and amino acids.Applied microbiology and biotechnology · 2007Review
- Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.Applied and environmental microbiology · 2006Article
- Engineering the genotype of Acinetobacter sp. strain ADP1 to enhance biosynthesis of cyanophycin.Applied and environmental microbiology · 2006Article
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4 authors.
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Abstract
Protamylasse is a residual compound occurring during the industrial production of starch from potatoes. It contains a variety of nutrients and all necessary minerals and could be used as a carbon, nitrogen, and energy source for the growth of bacteria and also for cyanophycin (CGP) biosynthesis. Media containing protamylasse as the sole compound diluted only in water were therefore examined for their suitability in CGP production. Among various bacterial strains investigated in this study, a recombinant strain of Escherichia coli DH1 harboring plasmid pMa/c5-914::cphA6803, which carries the cyanophycin synthetase structural gene (cphA) from Synechocystis sp. strain PCC6803, was found to be most suitable. Various cultivation conditions for high CGP contents were first optimized in shake flask cultures. The optimized conditions were then successfully applied to 30- and 500-liter fermentation scales in stirred tank reactors. A maximum CGP content of 28% (wt/wt) CGP per cell dry matter was obtained in 6% (vol/vol) protamylasse medium at an initial pH of 7.0 within a cultivation period of only 24 h. The CGP contents obtained with this recombinant strain employing protamylasse medium were higher than those obtained with the same strain cultivated in mineral salts medium or in expensive commercial complex media such as Luria-Bertani or Terrific broth. It was shown that most amino acids present in the protamylasse medium were almost completely utilized by the cells during cultivation. Exceptions were alanine, tryptophan, tyrosine, and most interestingly, arginine. Furthermore, CGP was easily isolated from protamylasse-grown cells by applying the acid extraction method. The CGP exhibited a molecular mass of about 26 to 30 kDa and was composed of 50% (mol/mol) aspartate, 46% (mol/mol) arginine, and 4% (mol/mol) lysine. The use of cheap residual protamylasse could contribute in establishing an economically and also ecologically feasible process for the biotechnological production of CGP.
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