ArticleProceedings of the National Academy of Sciences of the United States of America2005
Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 64 citations in OpenAlex.
- ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct.Cell reports · 2023Article
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- HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis.Nature communications · 2022Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins.Scientific reports · 2020Article
- Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila.FEBS letters · 2019Article
- Homozygous TAF8 mutation in a patient with intellectual disability results in undetectable TAF8 protein, but preserved RNA polymerase II transcription.Human molecular genetics · 2018Article
- Imaging of native transcription factors and histone phosphorylation at high resolution in live cells.The Journal of cell biology · 2018Article
- Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila.Cell cycle (Georgetown, Tex.) · 2018Article
- Different modes of enhancer-specific regulation by Runt and Even-skipped duringMolecular biology of the cell · 2017Article
- dTAF10- and dTAF10b-Containing Complexes Are Required for Ecdysone-Driven Larval-Pupal Morphogenesis in Drosophila melanogaster.PloS one · 2015Article
- Article
- The DUBm subunit Sgf11 is required for mRNA export and interacts with Cbp80 in Drosophila.Nucleic acids research · 2012Article
- Activator-induced spread of poly(ADP-ribose) polymerase promotes nucleosome loss at Hsp70.Molecular cell · 2012Article
- Pcaf modulates polyglutamine pathology in a Drosophila model of Huntington's disease.Neuro-degenerative diseases · 2012Article
- Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression.PloS one · 2012Article
- The NSL complex regulates housekeeping genes in Drosophila.PLoS genetics · 2012Article
- Inducible hsp70 in the regulation of cancer cell survival: analysis of chaperone induction, expression and activity.Cancers · 2011Article
- Drosophila Inducer of MEiosis 4 (IME4) is required for Notch signaling during oogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The Transition of Poised RNA Polymerase II to an Actively Elongating State Is a "Complex" Affair.Genetics research international · 2011Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The presence of general transcription factors and other coactivators at the Drosophila hsp70 gene promoter in vivo has been examined by polytene chromosome immunofluorescence and chromatin immunoprecipitation at endogenous heat-shock loci or at a hsp70 promoter-containing transgene. These studies indicate that the hsp70 promoter is already occupied by TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 and TRRAP), and the Mediator complex subunit 13 before heat shock. After heat shock, there is a significant recruitment of the heat-shock transcription factor, RNA polymerase II, XPD, GCN5, TRRAP, or Mediator complex 13 to the hsp70 promoter. Surprisingly, upon heat shock, there is a marked diminution in the occupancy of TBP, six different TAFs, TFIIB, and TFIIF, whereas there is no change in the occupancy of these factors at ecdysone-induced loci under the same conditions. Hence, these findings reveal a distinct mechanism of transcriptional induction at the hsp70 promoters, and further indicate that the apparent promoter occupancy of the general transcriptional factors does not necessarily reflect the transcriptional state of a gene.
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