ArticleProceedings of the National Academy of Sciences of the United States of America2005
Combining chemical genetics and proteomics to identify protein kinase substrates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 116 citations in OpenAlex.
- Redundant targeting of Isr1 by two CDKs in mitotic cells.Current genetics · 2021Review
- The kinase Isr1 negatively regulates hexosamine biosynthesis in S. cerevisiae.PLoS genetics · 2020Article
- Mapping proteome-wide targets of protein kinases in plant stress responses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Functional Analysis of Kinases and Transcription Factors inG3 (Bethesda, Md.) · 2017Article
- A computational protocol to evaluate the effects of protein mutants in the kinase gatekeeper position on the binding of ATP substrate analogues.BMC research notes · 2017Article
- Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response.Nature communications · 2016Article
- Lipid synthesis and membrane contact sites: a crossroads for cellular physiology.Journal of lipid research · 2016Review
- Phosphorylation of Yeast Pah1 Phosphatidate Phosphatase by Casein Kinase II Regulates Its Function in Lipid Metabolism.The Journal of biological chemistry · 2016Article
- Phosphorylation regulates the ubiquitin-independent degradation of yeast Pah1 phosphatidate phosphatase by the 20S proteasome.The Journal of biological chemistry · 2015Article
- Cross-talk phosphorylations by protein kinase C and Pho85p-Pho80p protein kinase regulate Pah1p phosphatidate phosphatase abundance in Saccharomyces cerevisiae.The Journal of biological chemistry · 2014Article
- TORC1 regulates Pah1 phosphatidate phosphatase activity via the Nem1/Spo7 protein phosphatase complex.PloS one · 2014Article
- Sucrose-induced receptor kinase SIRK1 regulates a plasma membrane aquaporin in Arabidopsis.Molecular & cellular proteomics : MCP · 2013Article
- Current technologies to identify protein kinase substrates in high throughput.Frontiers in biology · 2013Article
- Phosphatidate phosphatase, a key regulator of lipid homeostasis.Biochimica et biophysica acta · 2013Review
- A versatile mass spectrometry-based method to both identify kinase client-relationships and characterize signaling network topology.Journal of proteome research · 2013Article
- Yeast Trm7 interacts with distinct proteins for critical modifications of the tRNAPhe anticodon loop.RNA (New York, N.Y.) · 2012Article
- Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast.The Journal of biological chemistry · 2012Article
- Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics.Molecular & cellular proteomics : MCP · 2012Article
- Pho85p-Pho80p phosphorylation of yeast Pah1p phosphatidate phosphatase regulates its activity, location, abundance, and function in lipid metabolism.The Journal of biological chemistry · 2012Article
- Morphogenesis and the cell cycle.Genetics · 2012Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Phosphorylation is a ubiquitous protein modification important for regulating nearly every aspect of cellular biology. Protein kinases are highly conserved and constitute one of the largest gene families. Identifying the substrates of a kinase is essential for understanding its cellular role, but doing so remains a difficult task. We have developed a high-throughput method to identify substrates of yeast protein kinases that employs a collection of yeast strains each expressing a single epitope-tagged protein and a chemical genetic strategy that permits kinase reactions to be performed in native, whole-cell extracts. Using this method, we screened 4,250 strains expressing epitope-tagged proteins and identified 24 candidate substrates of the Pho85-Pcl1 cyclin-dependent kinase, including the known substrate Rvs167. The power of this method to identify true kinase substrates is strongly supported by functional overlap and colocalization of candidate substrates and the kinase, as well as by the specificity of Pho85-Pcl1 for some of the substrates compared with another Pho85-cyclin kinase complex. This method is readily adaptable to other yeast kinases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.