ArticleJournal of fluorescence2006
Epicocconone, a new cell-permeable long Stokes' shift fluorescent stain for live cell imaging and multiplexing.
Article in Journal of fluorescence, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 33 citations in OpenAlex.
- Bioinspired large Stokes shift small molecular dyes for biomedical fluorescence imaging.Science advances · 2022Article
- Plant-Fungal Interactions: A Case Study ofPlants (Basel, Switzerland) · 2020Article
- Color-Coded Super-Resolution Small-Molecule Imaging.Chembiochem : a European journal of chemical biology · 2016Article
- A theoretical study on diastereoselective oxidative dearomatization by iodoxybenzoic acid.Journal of molecular modeling · 2014Article
- Aurones: small molecule visible range fluorescent probes suitable for biomacromolecules.Journal of fluorescence · 2011Article
- Determination of sphingosine kinase activity for cellular signaling studies.Analytical chemistry · 2008Article
- Myristoyl-based transport of peptides into living cells.Biochemistry · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epicocconone is a heterocyclic natural product from the fungus Epicoccum nigrum that fluoresces weakly in the green (520 nm). However, cells exposed to epicocconone rapidly absorb the dye and become bright orange fluorescent because the natural product reacts reversibly with proteins. The orange fluorescence is enhanced in lipophilic environments, allowing the visualization of membranous organelles and lipid rafts but does not stain oligonucleotides. As the unconjugated dye has no orange fluorescence, there is no need to wash out the excess fluorophore. Epicocconone is a neutral, non-toxic, small molecule that appears to diffuse readily into live of fixed cells without the need for permeabilization. These features enable the real-time imaging of live cells and the study of organelle movements. Cells stained with epicocconone are excitable by common lasers (UV, 405, 488, and 532 nm) and its long Stokes' shift allows multiplexing applications with more common short Stokes' fluorophores using a single light source.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.