ArticleCurrent microbiology2005
Characterization of the CaENG1 gene encoding an endo-1,3-beta-glucanase involved in cell separation in Candida albicans.
Article in Current microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
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- The β-1,6-glucanase MoGlu16 is Required for Mycelia Growth and Pathogenicity of Magnaporthe oryzae.Current microbiology · 2025Article
- Antifungal Efficacy of 3D-Cultured Palatal Mesenchymal Stem Cells and Their Secreted Factors againstACS infectious diseases · 2025Article
- Protein kinase A signaling regulates immune evasion by shaving and concealing fungal β-1,3-glucan.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- The good, the bad, and the hazardous: comparative genomic analysis unveils cell wall features in the pathogen Candidozyma auris typical for both baker's yeast and Candida.FEMS yeast research · 2024Article
- Insect fungal pathogens secrete a cell wall-associated glucanase that acts to help avoid recognition by the host immune system.PLoS pathogens · 2023Article
- Nature of β-1,3-Glucan-Exposing Features on Candida albicans Cell Wall and Their Modulation.mBio · 2022Article
- Characterization ofMicroorganisms · 2022Article
- Control of β-glucan exposure by the endo-1,3-glucanase Eng1 in Candida albicans modulates virulence.PLoS pathogens · 2022Article
- Comparative Genomics of ThreeFrontiers in microbiology · 2022Article
- The Role ofFrontiers in cellular and infection microbiology · 2021Review
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- The Glucan-Remodeling Enzyme Phr1p and the Chitin Synthase Chs1p Cooperate to Maintain Proper Nuclear Segregation and Cell Integrity inFrontiers in cellular and infection microbiology · 2019Article
- The activity of bacterial peptidylarginine deiminase is important during formation of dual-species biofilm by periodontal pathogen Porphyromonas gingivalis and opportunistic fungus Candida albicans.Pathogens and disease · 2018Article
- Eng1 and Exg8 Are the Major β-Glucanases Secreted by the Fungal PathogenThe Journal of biological chemistry · 2017Article
- A computational model for regulation of nanoscale glucan exposure in Candida albicans.PloS one · 2017Article
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Candida albicans CaENG1 gene encoding an endo-1,3-beta-glucanase was cloned by screening a genomic library with a DNA probe obtained by polymerase chain reaction using synthetic oligonucleotides designed according to conserved regions found between two Saccharomyces cerevisiae endo-1,3-beta-glucanases (Eng1p and Eng2p). The gene contains a 3435-bp open reading frame (ORF), capable of encoding a protein of 1145 amino acids (124,157 Da), that contains no introns. Comparison of the ScEng1p sequence with partial C. albicans genomic sequences revealed the presence of a second protein with sequence similarity (the product of the Ca20C1.22c ORF, which was named CaENG2). Disruption of the CaENG1 gene in C. albicans had no dramatic effects on the growth rate of the strains, but it resulted in the formation of chains of cells, suggesting that the protein is involved in cell separation. Expression of CaENG1 in S. cerevisiae cells afforded a 12-fold increase in the 1,3-beta-glucanase activity detected in culture supernatants, showing that the protein has similar enzymatic activity to that of the S. cerevisiae Eng1p. In addition, when the C. albicans protein was expressed under its native promoter in S. cerevisiae eng1 mutant cells, it was able to complement the separation defect of this mutant, indicating that these two proteins are true functional homologues.
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