ArticleBiophysical journal2006
Lifetime measurements reveal kinetic differences between homophilic cadherin bonds.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Characterizing interactions in E-cadherin assemblages.Biophysical journal · 2023Article
- Engineering the Interactions of Classical Cadherin Cell-Cell Adhesion Proteins.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- A mesoscale mechanical model of cellular interactions.Biophysical journal · 2021Article
- Single-molecule force spectroscopy reveals the dynamic strength of the hair-cell tip-link connection.Nature communications · 2021Article
- Article
- Freezing shortens the lifetime of DNA molecules under tension.Journal of biological physics · 2017Article
- Tissue mechanics and adhesion during embryo development.Developmental biology · 2015Review
- Direct Detection of α-Synuclein Dimerization Dynamics: Single-Molecule Fluorescence Analysis.Biophysical journal · 2015Article
- Covalent and density-controlled surface immobilization of E-cadherin for adhesion force spectroscopy.PloS one · 2014Article
- β-Catenin serves as a clutch between low and high intercellular E-cadherin bond strengths.Biophysical journal · 2013Article
- Tuning the kinetics of cadherin adhesion.The Journal of investigative dermatology · 2013Review
- Article
- Cadherin recognition and adhesion.Current opinion in cell biology · 2012Review
- Cadherin point mutations alter cell sorting and modulate GTPase signaling.Journal of cell science · 2012Article
- Thinking outside the cell: how cadherins drive adhesion.Trends in cell biology · 2012Review
- Regulation of catch bonds by rate of force application.The Journal of biological chemistry · 2011Article
- Multiphoton flow cytometry to assess intrinsic and extrinsic fluorescence in cellular aggregates: applications to stem cells.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2011Article
- Tissue organization by cadherin adhesion molecules: dynamic molecular and cellular mechanisms of morphogenetic regulation.Physiological reviews · 2011Review
- Molecular mechanistic insights into the endothelial receptor mediated cytoadherence of Plasmodium falciparum-infected erythrocytes.PloS one · 2011Article
- Biomembrane force probe investigation of RNA dissociation.European biophysics journal : EBJ · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cadherins are multidomain adhesion proteins whose interactions direct cell sorting during histogenesis. They determine cell adhesion specificity, but prior studies failed to identify physical differences that could underlie cell sorting. These single molecule studies identify kinetic and strength differences between different cadherins. They further demonstrate that the modular extracellular architecture of cleavage stage C-cadherin supports a multistate binding mechanism. These multiple bonds exhibit a kinetic hierarchy of strengths that map to the different cadherin domains. The outer two N-terminal domains of C-cadherin form two bound states with dissociation rates of 3.9 and 0.02 s(-1). The latter is 25-fold slower than between the corresponding epithelial cadherin segments. In addition to the two fast bonds, the five-domain fragment (CEC1-5) forms two additional stronger, longer-lived bonds with dissociation rates of 0.00039 and 0.00001 s(-1). We further quantified the lifetimes of bonds subject to a constant force, and thus identified multiple dissociation events with rates that agree quantitatively with the force spectroscopy data. The qualitative features are similar to those reported for epithelial cadherin. However, the significant differences in the dissociation rates of the outer domains, which include the specificity-determining region, suggest that kinetic differences may determine cadherin discrimination, rather than adhesion energies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.