ArticleBiophysical journal2006
Weak force stalls protrusion at the leading edge of the lamellipodium.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- The Role of Mechanotransduction in Contact Inhibition of Locomotion and Proliferation.International journal of molecular sciences · 2024Review
- Keratocytes migrate against flow with a roly-poly-like mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Decomposition of cell activities revealing the role of the cell cycle in driving biofunctional heterogeneity.Scientific reports · 2021Article
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- Leukocyte transmigration and longitudinal forward-thrusting force in a microfluidic Transwell device.Biophysical journal · 2021Article
- A Bistable Mechanism Mediated by Integrins Controls Mechanotaxis of Leukocytes.Biophysical journal · 2020Article
- Mechanisms and in vivo functions of contact inhibition of locomotion.Nature reviews. Molecular cell biology · 2017Review
- Branching and capping determine the force-velocity relationships of branching actin networks.Physical biology · 2013Article
- Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.Biophysical journal · 2012Article
- The elementary events underlying force generation in neuronal lamellipodia.Scientific reports · 2011Article
- Force generation in lamellipodia is a probabilistic process with fast growth and retraction events.Biophysical journal · 2010Article
- Bipedal locomotion in crawling cells.Biophysical journal · 2010Article
- Growth factor regulation of corneal keratocyte differentiation and migration in compressed collagen matrices.Investigative ophthalmology & visual science · 2010Article
- Force transmission in migrating cells.The Journal of cell biology · 2010Article
- Mechanics of the F-actin cytoskeleton.Journal of biomechanics · 2010Review
- Actin dynamics: from nanoscale to microscale.Annual review of biophysics · 2010Review
- Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions.Journal of leukocyte biology · 2009Article
- Critical stresses for cancer cell detachment in microchannels.European biophysics journal : EBJ · 2009Article
- Modeling cell interactions under flow.Journal of mathematical biology · 2009Review
- Mathematics of cell motility: have we got its number?Journal of mathematical biology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Protrusion, the first step of cell migration, is driven by actin polymerization coupled to adhesion at the cell's leading edge. Polymerization and adhesive forces have been estimated, but the net protrusion force has not been measured accurately. We arrest the leading edge of a moving fish keratocyte with a hydrodynamic load generated by a fluid flow from a micropipette. The flow arrests protrusion locally as the cell approaches the pipette, causing an arc-shaped indentation and upward folding of the leading edge. The effect of the flow is reversible upon pipette removal and dependent on the flow direction, suggesting that it is a direct effect of the external force rather than a regulated cellular response. Modeling of the fluid flow gives a surprisingly low value for the arresting force of just a few piconewtons per micrometer. Enhanced phase contrast, fluorescence, and interference reflection microscopy suggest that the flow does not abolish actin polymerization and does not disrupt the adhesions formed before the arrest but rather interferes with weak nascent adhesions at the very front of the cell. We conclude that a weak external force is sufficient to reorient the growing actin network at the leading edge and to stall the protrusion.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.