ArticleNucleic acids research2005
Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP).
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Deep sequencing in library selection projects: what insight does it bring?Current opinion in structural biology · 2015Review
- From deep sequencing to actual clones.Protein engineering, design & selection : PEDS · 2014Article
- Navigating and mining modENCODE data.Methods (San Diego, Calif.) · 2014Review
- Inverse fusion PCR cloning.PloS one · 2012Article
- Experimental mapping of soluble protein domains using a hierarchical approach.Nucleic acids research · 2011Article
- An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences.PloS one · 2011Article
- Rapid interactome profiling by massive sequencing.Nucleic acids research · 2010Article
- Revisiting the protein-coding gene catalog of Drosophila melanogaster using 12 fly genomes.Genome research · 2007Article
- Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures.Nature · 2007Article
- Characterization of the aldo-keto reductase 1C gene cluster on pig chromosome 10: possible associations with reproductive traits.BMC veterinary research · 2006Article
- Viral metagenomics.Reviews in medical virologyReview
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5'- and 3'-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and proteins. Expressed Sequence Tag (EST) sequencing is the method of choice for recovering cDNAs representing many of the transcripts encoded in a eukaryotic genome. However, EST sequencing samples a cDNA library at random, and it recovers transcripts with low expression levels inefficiently. We describe a PCR-based method for directed screening of plasmid cDNA libraries. We demonstrate its utility in a screen of libraries used in our Drosophila EST projects for 153 transcription factor genes that were not represented by full-length cDNA clones in our Drosophila Gene Collection. We recovered high-quality, full-length cDNAs for 72 genes and variously compromised clones for an additional 32 genes. The method can be used at any scale, from the isolation of cDNA clones for a particular gene of interest, to the improvement of large gene collections in model organisms and the human. Finally, we discuss the relative merits of directed cDNA library screening and RT-PCR approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.