ArticleProceedings of the National Academy of Sciences of the United States of America2005
Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Determination of isoform-specific RNA structure with nanopore long reads.Nature biotechnology · 2021Article
- Regulation of class V myosin.Cellular and molecular life sciences : CMLS · 2018Review
- Melting temperature highlights functionally important RNA structure and sequence elements in yeast mRNA coding regions.Nucleic acids research · 2017Article
- RNA mNature · 2017Article
- Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.Nature structural & molecular biology · 2017Article
- Symmetry from Asymmetry or Asymmetry from Symmetry?Cold Spring Harbor symposia on quantitative biology · 2017Article
- Structure of a myosin•adaptor complex and pairing by cargo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A single molecule approach to mRNA transport by a class V myosin.RNA biology · 2014Article
- Here, there, everywhere. mRNA localization in budding yeast.RNA biology · 2014Review
- In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation.The Journal of cell biology · 2013Article
- Translation- and SRP-independent mRNA targeting to the endoplasmic reticulum in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Article
- Single-molecule reconstitution of mRNA transport by a class V myosin.Nature structural & molecular biology · 2013Article
- Assembly of mRNA-protein complexes for directional mRNA transport in eukaryotes--an overview.Current protein & peptide science · 2012Review
- Principles of mRNA transport in yeast.Cellular and molecular life sciences : CMLS · 2012Review
- Review
- CodingMotif: exact determination of overrepresented nucleotide motifs in coding sequences.BMC bioinformatics · 2012Article
- Control of cytoplasmic mRNA localization.Cellular and molecular life sciences : CMLS · 2012Review
- p180 promotes the ribosome-independent localization of a subset of mRNA to the endoplasmic reticulum.PLoS biology · 2012Article
- Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cytoplasmic mRNA localization is a mechanism used by many organisms to generate asymmetry and sequester protein activity. In the yeast Saccharomyces cerevisiae, mRNA transport to bud tips of dividing cells is mediated by the binding of She2p, She3p, and Myo4p to coding regions of the RNA. To date, 24 bud-localized mRNAs have been identified, yet the RNA determinants that mediate localization remain poorly understood. Here, we used nonhomologous random recombination to generate libraries of sequences that could be selected for their ability to bind She-complex proteins, thereby providing an unbiased approach for minimizing and mapping localization elements in several transported RNAs. Analysis of the derived sequences and predicted secondary structures revealed short sequence motifs that mediate binding to the She complex and RNA localization to the bud tip in vivo. A predicted single-stranded core CG dinucleotide appears to be an important component of the RNA-protein interface, although other nucleotides contribute in a context-dependent manner. Our findings further our understanding of RNA recognition by the She complex, and the methods used here should be applicable for elucidating minimal RNA motifs involved in many other types of interactions.
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