ArticleDevelopmental biology2006
Nucleoporins NPP-1, NPP-3, NPP-4, NPP-11 and NPP-13 are required for proper spindle orientation in C. elegans.
Article in Developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Ultrastructure Expansion Microscopy applied tomicroPublication biology · 2024Article
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Decreased mechanotransduction prevents nuclear collapse in aProceedings of the National Academy of Sciences of the United States of America · 2020Article
- C. elegans pronuclei fuse after fertilization through a novel membrane structure.The Journal of cell biology · 2020Article
- Dissecting the eQTL Micro-Architecture inFrontiers in genetics · 2020Article
- Channel Nucleoporins Recruit PLK-1 to Nuclear Pore Complexes to Direct Nuclear Envelope Breakdown in C. elegans.Developmental cell · 2017Article
- A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import.Molecular biology of the cell · 2015Article
- An RNAi-based suppressor screen identifies interactors of the Myt1 ortholog of Caenorhabditis elegans.G3 (Bethesda, Md.) · 2014Article
- Nup62: a novel regulator of centrosome integrity and function.Cell cycle (Georgetown, Tex.) · 2014Article
- The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos.Molecular biology of the cell · 2012Article
- Time-lapse microscopy of early embryogenesis in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2011Article
- NPP-16/Nup50 function and CDK-1 inactivation are associated with anoxia-induced prophase arrest in Caenorhabditis elegans.Molecular biology of the cell · 2010Article
- Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope.Journal of cell science · 2009Article
- Structure, dynamics and function of nuclear pore complexes.Trends in cell biology · 2008Review
- SPD-3 is required for spindle alignment in Caenorhabditis elegans embryos and localizes to mitochondria.Genetics · 2007Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Nucleoporins are components of the nuclear pore, which is required for nucleo-cytoplasmic transport. We report a role for a subclass of nucleoporins in orienting the mitotic spindle in C. elegans embryos. RNAi-mediated depletion of any of five putative nucleoporins npp-1, npp-3, npp-4, npp-11, and npp-13 leads to indistinguishable spindle orientation defects. Transgenic worms expressing NPP-1::GFP or NPP-11::GFP show GFP localization at the nuclear envelope, consistent with their predicted function. NPP-1 interacts with the other nucleoporins in yeast two-hybrid assays, suggesting that the proteins affect spindle orientation by a common process. The failed orientation phenotype of npp-1(RNAi) is at least partially epistatic to the ectopic spindle rotation in the AB blastomere of par-3 mutant embryos. This suggests that NPP-1 contributes to the mechanics of spindle orientation. However, NPP-1 is also required for PAR-6 asymmetry at the two-cell stage, indicating that nucleoporins may be required to define cortical domains in the germ line blastomere P1. Nuclear envelope structure is abnormal in npp-1(RNAi) embryos, but the envelope maintains its integrity, and most nuclear proteins we assayed accumulate normally. These findings raise the possibility that these nucleoporins may have direct roles in orienting the mitotic spindle and the maintenance of cell polarity.
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