ArticlePlant cell reports2006
Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems.Heliyon · 2024Review
- Molecular farming expression of recombinant fusion proteins applied to skincare strategies.PeerJ · 2024Review
- Genetic Containment for Molecular Farming.Plants (Basel, Switzerland) · 2022Review
- Current advances and future prospects in production of recombinant insulin and other proteins to treat diabetes mellitus.Biotechnology letters · 2022Review
- Oral delivery of therapeutic proteins bioencapsulated in plant cells: preclinical and clinical advances.Current opinion in colloid & interface science · 2021Article
- Oral Administration of Silkworm-Produced GAD65 and Insulin Bi-Autoantigens against Type 1 Diabetes.PloS one · 2016Article
- Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV.Plant biotechnology journal · 2015Article
- Evaluation of somatic embryos of alfalfa for recombinant protein expression.Plant cell reports · 2015Article
- Production of a plant-derived immunogenic protein targeting ApoB100 and CETP: toward a plant-based atherosclerosis vaccine.Molecular biotechnology · 2014Article
- Expression of recombinant vaccines and antibodies in plants.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Review
- Expression of recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi.Molecular biology reports · 2013Article
- Recent advances and safety issues of transgenic plant-derived vaccines.Applied microbiology and biotechnology · 2013Review
- Two decades of plant-based candidate vaccines: a review of the chimeric protein approaches.Plant cell reports · 2011Review
- Expression of a cholera toxin B subunit-neutralizing epitope of the porcine epidemic diarrhea virus fusion gene in transgenic lettuce (Lactuca sativa L.).Molecular biotechnology · 2011Article
- Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide.Plant biotechnology journal · 2011Article
- High-yield expression of recombinant soybean agglutinin in plants using transient and stable systems.Transgenic research · 2011Article
- Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues.Molecular biotechnology · 2010Article
- High level expression of a functionally active cholera toxin B: rabies glycoprotein fusion protein in tobacco seeds.Plant cell reports · 2009Article
- Expression of cholera toxin B subunit in transgenic rice endosperm.Molecular biotechnology · 2008Article
- Expression of modified gene encoding functional human alpha-1-antitrypsin protein in transgenic tomato plants.Transgenic research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A DNA construct containing the cholera toxin B subunit (CTB) gene genetically fused to a nucleotide sequence encoding three copies of tandemly repeated diabetes-associated autoantigen, the B chain of human insulin, was produced and transferred into low-nicotine tobaccos by Agrobacterium. Integration of the fusion gene into the plant genome was confirmed by polymerase chain reaction (PCR). The results of immunoblot analysis verified the synthesis and assembly of the fusion protein into pentamers in transgenic tobacco. GM1-ELISA showed that the plant-derived fusion protein retained GM1-ganglioside receptor binding specificity. The fusion protein accounted for 0.11% of the total leaf protein. The production of transgenic plants expressing CTB-InsB3 offers a new opportunity to test plant-based oral antigen therapy against autoimmune diabetes by inducing oral tolerance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.