ArticlePLoS pathogens2005
Comparative signature-tagged mutagenesis identifies Pseudomonas factors conferring resistance to the pulmonary collectin SP-A.
Article in PLoS pathogens, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.Journal of bacteriology · 2026Review
- Quorum sensing regulation by the nitrogen phosphotransferase system inJournal of bacteriology · 2025Article
- Article
- Mammals' humoral immune proteins and peptides targeting the bacterial envelope: from natural protection to therapeutic applications against multidrug-resistant Gram-negatives.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Cooperative action of SP-A and its trimeric recombinant fragment with polymyxins against Gram-negative respiratory bacteria.Frontiers in immunology · 2022Article
- Article
- Random Peptide Mixtures as Safe and Effective Antimicrobials againstACS infectious diseases · 2021Article
- Surfactant phospholipids act as molecular switches for premature induction of quorum sensing-dependent virulence inVirulence · 2020Article
- The Role of Collectins and Galectins in Lung Innate Immune Defense.Frontiers in immunology · 2018Review
- Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection.Infection and immunity · 2016Article
- A Putative ABC Transporter Permease Is Necessary for Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa under Aerobic and Anaerobic Planktonic and Biofilm Conditions.Frontiers in microbiology · 2016Article
- Natural Anti-Infective Pulmonary Proteins: In Vivo Cooperative Action of Surfactant Protein SP-A and the Lung Antimicrobial Peptide SP-BN.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Dephosphorylated NPr is involved in an envelope stress response of Escherichia coli.Microbiology (Reading, England) · 2015Article
- Type IV pilus glycosylation mediates resistance of Pseudomonas aeruginosa to opsonic activities of the pulmonary surfactant protein A.Infection and immunity · 2015Article
- Type IV pilus of Pseudomonas aeruginosa confers resistance to antimicrobial activities of the pulmonary surfactant protein-A.Journal of innate immunity · 2014Article
- Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.Molecular microbiology · 2013Article
- Repurposing the antimycotic drug flucytosine for suppression of Pseudomonas aeruginosa pathogenicity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Pulmonary infection by Yersinia pestis rapidly establishes a permissive environment for microbial proliferation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The Pseudomonas aeruginosa flagellum confers resistance to pulmonary surfactant protein-A by impacting the production of exoproteases through quorum-sensing.Molecular microbiology · 2011Article
- Pseudomonas aeruginosa elastase provides an escape from phagocytosis by degrading the pulmonary surfactant protein-A.PloS one · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The pulmonary collectin, surfactant protein A (SP-A), is a broad spectrum opsonin with microbicidal membrane permeabilization properties that plays a role in the innate immune response of the lung. However, the factors that govern SP-A's microbial specificity and the mechanisms by which it mediates membrane permeabilization and opsonization are not fully understood. In an effort to identify bacterial factors that confer susceptibility or resistance to SP-A, we used comparative signature-tagged mutagenesis to screen a library of 1,680 Pseudomonas aeruginosa mutants for evidence of differential pulmonary clearance in SP-A-sufficient (SP-A) and SP-A-deficient (SP-A) mice. Two SP-A-sensitive P. aeruginosa mutants harboring transposon insertions in genes required for salicylate biosynthesis (pch) and phosphoenolpyruvate-protein-phosphotransferase (ptsP) were recovered. The mutants were indistinguishable from the parental wild-type PA01 with regard to opsonization by SP-A, but they exhibited increased susceptibility to SP-A-mediated membrane permeabilization. These results suggest that bacterial gene functions that are required to maintain membrane integrity play crucial roles in resistance of P. aeruginosa to the permeabilizing effects of SP-A.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.