ArticleGenetics2006
The purine synthesis gene Prat2 is required for Drosophila metamorphosis, as revealed by inverted-repeat-mediated RNA interference.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Dynamic morphological staging of Drosophila pupal development.Scientific reports · 2026Article
- Functional characterization of novel NPRL3 mutations identified in three families with focal epilepsy.Science China. Life sciences · 2023Article
- De novo purine nucleotide biosynthesis mediated by MoAde4 is required for conidiation, host colonization and pathogenicity in Magnaporthe oryzae.Applied microbiology and biotechnology · 2022Article
- Article
- Visualization of insect metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- A Genetic Screen Using theG3 (Bethesda, Md.) · 2019Article
- Purine Homeostasis Is Necessary for Developmental Timing, Germline Maintenance and Muscle Integrity inGenetics · 2019Article
- The 'dance' of life: visualizing metamorphosis during pupation in the blow flyRoyal Society open science · 2017Article
- The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.Chemical communications (Cambridge, England) · 2013Article
- Article
- Uracil-containing DNA in Drosophila: stability, stage-specific accumulation, and developmental involvement.PLoS genetics · 2012Article
- A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster.Genetics · 2011Article
- Expression pattern diversity and functional conservation between retroposed PRAT genes from Drosophila melanogaster and Drosophila virilis.Journal of molecular evolution · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PRAT (phosphoribosylamidotransferase; E.C. 2.4.2.14) catalyzes the first reaction in de novo purine nucleotide biosynthesis. In Drosophila melanogaster, the Prat and Prat2 genes are both highly conserved with PRAT sequences from prokaryotes and eukaryotes. However, Prat2 organization and expression during development is different from Prat. We used RNA interference (RNAi) to knock down expression of both Prat and Prat2 to investigate their functions. Using the GAL4-UAS system, Prat RNAi driven by Act5c-GAL4 or tubP-GAL4 causes variable pupal lethality (48-100%) and approximately 50% female sterility, depending on the transgenic strains and drivers used. This observation agrees with the phenotype previously observed for Prat EMS-induced mutations. Prat2 RNAi driven by Act5C-GAL4 or tubP-GAL4 also results in variable pupal lethality (61-93%) with the different transgenic strains, showing that Prat2 is essential for fly development. However, Prat2 RNAi-induced arrested pupae have a head eversion defect reminiscent of the "cryptocephal" phenotype, whereas Prat RNAi-induced arrested pupae die later as pharate adults. We conclude that Prat2 is required during the prepupal stage while Prat is more important for the pupal stage. In addition, Prat and Prat2 double RNAi results in more severe pupal lethal phenotypes, suggesting that Prat and Prat2 have partially additive functions during Drosophila metamorphosis.
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