ArticleThe Journal of physiology2006
Synaptobrevin2-expressing vesicles in rat astrocytes: insights into molecular characterization, dynamics and exocytosis.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed.
- A Journey between Neurons and Astrocytes: A Tribute to Giorgio Carmignoto.Neurochemical research · 2025Article
- Molecular Motors in Blood-Brain Barrier Maintenance by Astrocytes.Brain sciences · 2025Review
- Gene Expression at the Tripartite Synapse: Bridging the Gap Between Neurons and Astrocytes.Advances in neurobiology · 2024Review
- The Key Role of Astrocytes in Amyotrophic Lateral Sclerosis and Their Commitment to Glutamate Excitotoxicity.International journal of molecular sciences · 2023Review
- Amyloid pathology disrupts gliotransmitter release in astrocytes.PLoS computational biology · 2022Article
- Revealing the contribution of astrocytes to glutamatergic neuronal transmission.Frontiers in cellular neuroscience · 2022Review
- Imaging Synaptic Density: The Next Holy Grail of Neuroscience?Frontiers in neuroscience · 2022Review
- Astrocyte arborization enhances CaGlia · 2021Article
- Exocytosis in Astrocytes.Biomolecules · 2021Review
- Gliocrine System: Astroglia as Secretory Cells of the CNS.Advances in experimental medicine and biology · 2019Review
- Exploring the Role of Astroglial Glutamate Release and Association With Synapses in Neuronal Function and Behavior.Biological psychiatry · 2018Review
- TNFα and IL-1β modify the miRNA cargo of astrocyte shed extracellular vesicles to regulate neurotrophic signaling in neurons.Cell death & disease · 2018Article
- Multiple Lines of Evidence Indicate That Gliotransmission Does Not Occur under Physiological Conditions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Gliotransmission: Beyond Black-and-White.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Review
- Physiology of Astroglia.Physiological reviews · 2018Review
- Astrocytes control synaptic strength by two distinct v-SNARE-dependent release pathways.Nature neuroscience · 2017Article
- Astroglial Vesicular Trafficking in Neurodegenerative Diseases.Neurochemical research · 2017Article
- Homeostasis of the Intraparenchymal-Blood Glutamate Concentration Gradient: Maintenance, Imbalance, and Regulation.Frontiers in molecular neuroscience · 2017Review
- Amyloid-β Impairs Vesicular Secretion in Neuronal and Astrocyte Peptidergic Transmission.Frontiers in molecular neuroscience · 2017Article
- A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.The Journal of general physiology · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SNARE-dependent exocytosis of glutamate-containing vesicles in astrocytes is increasingly viewed as an important signal at the basis of the astrocyte-to-neurone communication system in the brain. Here we provide further insights into the molecular features and dynamics of vesicles in cultured astrocytes. We found that immunoisolated synaptobrevin2 vesicles are clear vesicles quite heterogenous in size and contain the vesicular glutamate transporter v-Glut-2. Moreover, they are immunopositive for synaptotagmin IV, for AMPA receptor subunits GluR2,3 and, to a lesser extent, for GluR1. We also provide direct evidence for the functional expression of v-Glut-2 in astrocytes and demonstrate that synaptobrevin2-positive vesicles can specifically take up (3H)L-glutamate via a bafilomycin-sensitive mechanism. Finally, by time lapse confocal microscopy, we show that a subpopulation of vesicles (tagged with a synaptobrevin2-EGFP chimera) is highly mobile and can fuse with the plasma membrane, preferentially at the level of the astrocyte processes, in a Ca2+-dependent manner. These latter observations, together with the evidence reported here for the expression of functional v-Glut-2 in synaptobrevin2-positive vesicles, provide a molecular basis for regulated exocytosis in astrocyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.