ArticleThe Journal of physiology2006
Claudin-8 modulates paracellular permeability to acidic and basic ions in MDCK II cells.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 85 citations in OpenAlex.
- Claudins in kidney health and disease.Kidney research and clinical practice · 2022Article
- The kidney anion exchanger 1 affects tight junction properties via claudin-4.Scientific reports · 2019Article
- Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation.Annals of the New York Academy of Sciences · 2017Review
- One gene, two paracellular ion channels-claudin-10 in the kidney.Pflugers Archiv : European journal of physiology · 2017Review
- Tight junctions in pulmonary epithelia during lung inflammation.Pflugers Archiv : European journal of physiology · 2017Review
- Gluten-induced symptoms in diarrhea-predominant irritable bowel syndrome are associated with increased myosin light chain kinase activity and claudin-15 expression.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Role of CFTR in epithelial physiology.Cellular and molecular life sciences : CMLS · 2017Review
- Intestinal microbiota: a potential target for the treatment of postmenopausal osteoporosis.Bone research · 2017Review
- Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats.PloS one · 2017Article
- Tight junctions in inflammatory bowel diseases and inflammatory bowel disease associated colorectal cancer.World journal of gastroenterology · 2016Review
- Claudins and the kidney.Journal of the American Society of Nephrology : JASN · 2015Review
- In vitro transport characteristics of EFdA, a novel nucleoside reverse transcriptase inhibitor using Caco-2 and MDCKII cell monolayers.European journal of pharmacology · 2014Article
- Regulation of paracellular permeability: factors and mechanisms.Molecular biology reports · 2013Review
- Claudins and the modulation of tight junction permeability.Physiological reviews · 2013Review
- Claudin-17 forms tight junction channels with distinct anion selectivity.Cellular and molecular life sciences : CMLS · 2012Article
- Polystyrene nanoparticle trafficking across MDCK-II.Nanomedicine : nanotechnology, biology, and medicine · 2011Article
- Differential effects of claudin-3 and claudin-4 on alveolar epithelial barrier function.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Omeprazole decreases magnesium transport across Caco-2 monolayers.World journal of gastroenterology · 2011Article
- Epithelial remodeling and claudin mRNA abundance in the gill and kidney of puffer fish (Tetraodon biocellatus) acclimated to altered environmental ion levels.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2011Article
- Tight junction pore and leak pathways: a dynamic duo.Annual review of physiology · 2011Review
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Renal net acid excretion requires tubular reabsorption of filtered bicarbonate, followed by secretion of protons and ammonium in the collecting duct, generating steep transtubular gradients for these ions. To prevent passive backleak of these ions, the tight junctions in the collecting duct must be highly impermeable to these ions. We previously generated a Madin-Darby canine kidney (MDCK II) cell line with inducible expression of claudin-8, a tight junction protein expressed in the collecting duct. In these cells, claudin-8 was shown to function as a paracellular barrier to alkali metal and divalent cations. We have now used this model to test the hypothesis that claudin-8 also functions as a paracellular barrier to acidic or basic ions involved in renal acid excretion. We developed a series of precise and unbiased methods, based on a combination of diffusion potential, short-circuit current, and pH stat measurements, to estimate paracellular permeability to protons, ammonium and bicarbonate in MDCK II cells. We found that under control conditions (i.e. in the absence of claudin-8), these cells are highly permeable to the acidic and basic ions tested. Interestingly, proton permeation exhibited an unusually low activation energy similar to that in bulk solution. This suggests that paracellular proton transfer may occur by a Grotthuss mechanism, implying that the paracellular pores are sufficiently wide to accommodate water molecules in a freely mobile state. Induction of claudin-8 expression reduces permeability not only to protons, but also to ammonium and bicarbonate. We conclude that claudin-8 probably functions to limit the passive leak of these three ions via paracellular routes, thereby playing a permissive role in urinary net acid excretion.
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