ArticleThe Journal of physiology2006
Water transport by Na+-coupled cotransporters of glucose (SGLT1) and of iodide (NIS). The dependence of substrate size studied at high resolution.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- CSF hyperdynamics in rats mimicking the obesity and androgen excess characteristic of patients with idiopathic intracranial hypertension.Fluids and barriers of the CNS · 2024Article
- Toward New AQP4 Inhibitors: ORI-TRN-002.International journal of molecular sciences · 2024Article
- Non-Aquaporin Water Channels.Advances in experimental medicine and biology · 2023Article
- Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited.Fluids and barriers of the CNS · 2022Review
- The digestive tract as an essential organ for water acquisition in marine teleosts: lessons from euryhaline eels.Zoological letters · 2021Review
- Inter-species variation in monovalent anion substrate selectivity and inhibitor sensitivity in the sodium iodide symporter (NIS).PloS one · 2020Article
- Evaluating the involvement of cerebral microvascular endothelial NaJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Structural and functional significance of water permeation through cotransporters.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- ClEuropean biophysics journal : EBJ · 2016Article
- Relationship between TMEM16A/anoctamin 1 and LRRC8A.Pflugers Archiv : European journal of physiology · 2016Article
- Aquaporin 4 as a NH3 Channel.The Journal of biological chemistry · 2016Article
- Isoform-specific phosphorylation-dependent regulation of connexin hemichannels.Journal of neurophysiology · 2015Article
- TRPV4 and AQP4 Channels Synergistically Regulate Cell Volume and Calcium Homeostasis in Retinal Müller Glia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Vasopressin receptors V1a and V2 are not osmosensors.Physiological reports · 2015Article
- Aquaporin 5 knockout mouse lens develops hyperglycemic cataract.Biochemical and biophysical research communications · 2013Article
- Osmotic water transport in aquaporins: evidence for a stochastic mechanism.The Journal of physiology · 2013Article
- Transient formation of water-conducting states in membrane transporters.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Glial K⁺ clearance and cell swelling: key roles for cotransporters and pumps.Neurochemical research · 2012Review
- Cotransport of water by Na⁺-K⁺-2Cl⁻ cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2.The Journal of physiology · 2012Article
- Osmoregulation and epithelial water transport: lessons from the intestine of marine teleost fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relation between substrate and water transport was studied in Na+-coupled cotransporters of glucose (SGLT1) and of iodide (NIS) expressed in Xenopus oocytes. The water transport was monitored from changes in oocyte volume at a resolution of 20 pl, more than one order of magnitude better than previous investigations. The rate of cotransport was monitored as the clamp current obtained from two-electrode voltage clamp. The high resolution data demonstrated a fixed ratio between the turn-over of the cotransporter and the rate of water transport. This applied to experiments in which the rate of cotransport was changed by isosmotic application of substrate, by rapid changes in clamp voltage, or by poisoning. Transport of larger substrates gave rise to less water transport. For the rabbit SGLT1, 378+/-20 (n=18 oocytes) water molecules were cotransported along with the 2 Na+ ions and the glucose-analogue alpha-MDG (MW 194); using the larger sugar arbutin (MW 272) this number was reduced by a factor of at least 0.86+/-0.03 (15). For the human SGLT1 the respective numbers were 234+/-12 (18) and 0.85+/-0.8 (7). For NIS, 253+/-16 (12) water molecules were cotransported for each 2 Na+ and 1 thiocyanate (SCN-, MW 58), with I- as anion (MW 127) only 162+/-11 (19) water molecules were cotransported. The effect of substrate size suggests a molecular mechanism for water cotransport and is opposite to what would be expected from unstirred layer effects. Data were analysed by a model which combined cotransport and osmosis at the membrane with diffusion in the cytoplasm. The combination of high resolution measurements and precise modelling showed that water transport across the membrane can be explained by cotransport of water in the membrane proteins and that intracellular unstirred layers effects are minute.
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