ArticleJournal of bacteriology2005
Genetic characterization of Pseudomonas fluorescens SBW25 rsp gene expression in the phytosphere and in vitro.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Stable, fluorescent markers for tracking synthetic communities and assembly dynamics.Microbiome · 2024Article
- Learning perturbation-inducible cell states from observability analysis of transcriptome dynamics.Nature communications · 2023Article
- Revealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiology.Biodesign research · 2023Article
- Bridging the Gap: Type III Secretion Systems in Plant-Beneficial Bacteria.Microorganisms · 2022Article
- Metabolic Interactions betweenmSystems · 2021Article
- Characterization of the SPI-1 Type III Secretion System inFrontiers in microbiology · 2021Article
- Framework for Quantification of the Dynamics of Root Colonization byFrontiers in microbiology · 2020Article
- Type III Secretion System of Beneficial RhizobacteriaFrontiers in microbiology · 2019Article
- Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species.Scientific reports · 2018Article
- Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of Biofilm Formation.Frontiers in microbiology · 2018Article
- Quantitative Expression Analysis of SpA, FnbA and Rsp Genes in Staphylococcus aureus: Actively Associated in the Formation of Biofilms.Current microbiology · 2017Article
- Exploring the genomic traits of fungus-feeding bacterial genus Collimonas.BMC genomics · 2015Article
- Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24.Frontiers in plant science · 2015Article
- Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans.Clinical microbiology reviews · 2014Review
- Commonalities and differences of T3SSs in rhizobia and plant pathogenic bacteria.Frontiers in plant science · 2014Review
- Comparative genomics of plant-associated Pseudomonas spp.: insights into diversity and inheritance of traits involved in multitrophic interactions.PLoS genetics · 2012Article
- An improved, high-quality draft genome sequence of the Germination-Arrest Factor-producing Pseudomonas fluorescens WH6.BMC genomics · 2010Article
- Evolutionary history of the phl gene cluster in the plant-associated bacterium Pseudomonas fluorescens.Applied and environmental microbiology · 2009Article
- Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens.Genome biology · 2009Article
- Mining the genomes of plant pathogenic bacteria: how not to drown in gigabases of sequence.Molecular plant pathology · 2008Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant-colonizing Pseudomonas fluorescens strain SBW25 harbors a gene cluster (rsp) whose products show similarity to type III protein secretion systems found in plant and animal pathogens. Here we report a detailed analysis of the expression and regulation of the P. fluorescens rsp pathway, both in the phytosphere and in vitro. A combination of chromosomally integrated transcriptional reporter fusions, overexpressed regulatory genes, and specific mutants reveal that promoters controlling expression of rsp are actively transcribed in the plant rhizosphere but not (with the exception of the rspC promoter) in the phyllosphere. In synthetic medium, regulatory (rspL and rspR) and structural (rspU, plus the putative effector ropE) genes are poorly expressed; the rspC promoter is subject to an additional level of regulatory control. Ectopic expression of regulatory genes in wild-type and mutant backgrounds showed that RspR controls transcription of the alternate sigma factor, rspL, and that RspL controls expression of gene clusters encoding structural genes. Mutation of rspV did not affect RspR-mediated expression of rspU. A search for additional regulators revealed two candidates--one with a role in the conversion of alanine to pyruvate--suggesting that expression of rsp is partly dependent upon the metabolic status of the cell. Mutations in rsp regulators resulted in a significant reduction in competitive colonization of the root tips of sugar beet seedlings but also caused a marked increase in the lag phase of laboratory-grown cultures, indicating that rsp regulatory genes play a more significant general role in the function of P. fluorescens SBW25 than previously appreciated.
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