ArticleJournal of bacteriology2005
The ClgR protein regulates transcription of the clpP operon in Bifidobacterium breve UCC 2003.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Degradation Mechanism of AAA+ Proteases and Regulation ofBiomolecules · 2022Review
- Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.Applied and environmental microbiology · 2022Article
- Article
- Murein lytic enzyme TgaA of Bifidobacterium bifidum MIMBb75 modulates dendritic cell maturation through its cysteine- and histidine-dependent amidohydrolase/peptidase (CHAP) amidase domain.Applied and environmental microbiology · 2014Article
- DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks.Cold Spring Harbor perspectives in biology · 2013Review
- Article
- A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2012Article
- Analysis of predicted carbohydrate transport systems encoded by Bifidobacterium bifidum PRL2010.Applied and environmental microbiology · 2012Article
- Article
- Global genome transcription profiling of Bifidobacterium bifidum PRL2010 under in vitro conditions and identification of reference genes for quantitative real-time PCR.Applied and environmental microbiology · 2011Article
- ClpR protein-like regulator specifically recognizes RecA protein-independent promoter motif and broadly regulates expression of DNA damage-inducible genes in mycobacteria.The Journal of biological chemistry · 2011Article
- Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again.Microbial cell factories · 2011Review
- How do bifidobacteria counteract environmental challenges? Mechanisms involved and physiological consequences.Genes & nutrition · 2011Article
- Genomic insights into bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2010Review
- Characterization of a Clp protease gene regulator and the reaeration response in Mycobacterium tuberculosis.PloS one · 2010Article
- An interactive regulatory network controls stress response in Bifidobacterium breve UCC2003.Journal of bacteriology · 2009Article
- Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice.BMC microbiology · 2008Article
- Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum.Microbiology and molecular biology reviews : MMBR · 2007Review
- Molecular characterization of hsp20, encoding a small heat shock protein of bifidobacterium breve UCC2003.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five clp genes (clpC, clpB, clpP1, clpP2, and clpX), representing chaperone- and protease-encoding genes, were previously identified in Bifidobacterium breve UCC 2003. In the present study, we characterize the B. breve UCC 2003 clpP locus, which consists of two paralogous genes, designated clpP1 and clpP2, whose deduced protein products display significant similarity to characterized ClpP peptidases. Transcriptional analyses showed that the clpP1 and clpP2 genes are transcribed in response to moderate heat shock as a bicistronic unit with a single promoter. The role of a clgR homologue, known to control the regulation of clpP gene expression in Streptomyces lividans and Corynebacterium glutamicum, was investigated by gel mobility shift assays and DNase I footprint experiments. We show that ClgR, which in its purified form appears to exist as a dimer, requires a proteinaceous cofactor to assist in specific binding to a 30-bp region of the clpP promoter region. In pull-down experiments, a 56-kDa protein copurified with ClgR, providing evidence that the two proteins also interact in vivo and that the copurified protein represents the cofactor required for ClgR activity. The prediction of the ClgR three-dimensional structure provides further insights into the binding mode of this protein to the clpP1 promoter region and highlights the key amino acid residues believed to be involved in the protein-DNA interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.