ArticleBMC cell biology2005
Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures.
Article in BMC cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 58 citations in OpenAlex.
- Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-ProducingMicroorganisms · 2023Article
- Establishment and characterization of an immortalized bovine intestinal epithelial cell line.Journal of animal science · 2023Article
- Establishment of bovine 3D enteroid-derived 2D monolayers.Veterinary research · 2022Article
- First Metabolic Insights intoBiology · 2021Article
- Organoids: a promising new in vitro platform in livestock and veterinary research.Veterinary research · 2021Review
- Assessing Intestinal Health.Frontiers in veterinary science · 2021Review
- Lactoferrin translocates to the nucleus of bovine rectal epithelial cells in the presence of Escherichia coli O157:H7.Veterinary research · 2019Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- Developing a 3D intestinal epithelium model for livestock species.Cell and tissue research · 2019Article
- Establishment of an immortalized intestinal epithelial cell line from tree shrews by lentivirus-mediated hTERT gene transduction.Cytotechnology · 2019Article
- Short-Chain Fatty Acids Regulate the Immune Responses via G Protein-Coupled Receptor 41 in Bovine Rumen Epithelial Cells.Frontiers in immunology · 2019Article
- Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Immunobiotics for the Bovine Host: Their Interaction with Intestinal Epithelial Cells and Their Effect on Antiviral Immunity.Frontiers in immunology · 2018Review
- Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Diagnostic and Research Aspects of Small Intestinal Disaccharidases in Coeliac Disease.Journal of immunology research · 2017Review
- Investigation of JAKs/STAT-3 in lipopolysaccharide-induced intestinal epithelial cells.Clinical and experimental immunology · 2016Article
- Contribution of in vitro comparison of colorectal carcinoma cells from primary and metastatic lesions to elucidation of mechanisms of tumor progression and response to anticancer therapy.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation.Wiener klinische Wochenschrift · 2015Article
- Allelic variation contributes to bacterial host specificity.Nature communications · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract.
resultsCultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium.
conclusionThe present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations.
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