ArticlePlanta2006
High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 136 citations in OpenAlex.
- FvGI-FvCO-FvFT1 pathway activates FvTFL1 in long days to repress flowering in woodland strawberry (Fragaria vesca).The New phytologist · 2026Article
- FabHLH130-FaSPS1/FaINV positively contributes to the sugar accumulation in strawberry fruits.BMC plant biology · 2026Article
- Jasmonate Modulates Strawberry Susceptibility to Anthracnose by Activating SnRK2.1 to Regulate the WRKY50-JAZ5 Module.Plant biotechnology journal · 2026Article
- A signaling pathway mediating abscisic acid-induced jasmonic acid biosynthesis during strawberry ripening.Journal of integrative plant biology · 2026Article
- The role ofHorticulture research · 2025Article
- Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca.Scientific reports · 2025Article
- The FvABF3-FvALKBH10B-FvSEP3 cascade regulates fruit ripening in strawberry.Nature communications · 2024Article
- Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4.Plant methods · 2024Article
- High-temperature stress in strawberry: understanding physiological, biochemical and molecular responses.Planta · 2024Review
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening.The Plant cell · 2023Article
- Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry.Plant methods · 2023Article
- Article
- Comparative Transcriptome Analysis Reveals Novel Candidate Resistance Genes Involved in Defence againstInternational journal of molecular sciences · 2023Article
- TEMPRANILLO homologs in apple regulate flowering time in the woodland strawberry Fragaria vesca.Scientific reports · 2023Article
- FvNST1b NAC Protein Induces Secondary Cell Wall Formation in Strawberry.International journal of molecular sciences · 2022Article
- Chromosome-level genome assembly of the diploid blueberry Vaccinium darrowii provides insights into its subtropical adaptation and cuticle synthesis.Plant communications · 2022Article
- FvWRKY48 binds to the pectate lyase FvPLA promoter to control fruit softening in Fragaria vesca.Plant physiology · 2022Article
- Low temperature inhibits anthocyanin accumulation in strawberry fruit by activating FvMAPK3-induced phosphorylation of FvMYB10 and degradation of Chalcone Synthase 1.The Plant cell · 2022Article
- The protein kinase FvRIPK1 regulates plant morphogenesis by ABA signaling using seed genetic transformation in strawberry.Frontiers in plant science · 2022Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fragaria vesca L., a diploid (2n = 2x = 14) relative of the commercial octoploid strawberry, is an attractive model for functional genomics research in Rosaceae. Its small genome size, short reproductive cycle, and facile vegetative and seed propagation make F. vesca a promising candidate for forward and reverse genetics experiments. However, the lack of a high-efficiency transformation protocol required for systematic production of thousands of T-DNA insertional mutant lines and high-throughput gene validation is a major bottleneck. We describe a new transformation procedure that uses leaf explants from newly unfolded trifoliate leaves obtained from stock plants 6-7 weeks after seed germination, co-cultivation with Agrobacterium strain GV3101, and stringent selection on MS medium containing 4 mg l(-1) hygromycin. Using this protocol we achieved 100% transformation efficiency for 6 of 14 F. vesca accessions tested. Accession PI 551572 was determined to be the best candidate for a model in F. vesca functional genomics research, as it showed the greatest propensity for callus formation, transformation, shoot regeneration, ex vitro establishment, and plant growth, requiring only 14-15 weeks to complete its life cycle in different seasons in the greenhouse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.