ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Functional organization of a Schwann cell enhancer.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Transcriptional regulators of the Golli/myelin basic protein locus integrate additive and stealth activities.PLoS genetics · 2020Article
- Fortilin potentiates the peroxidase activity of Peroxiredoxin-1 and protects against alcohol-induced liver damage in mice.Scientific reports · 2016Article
- Article
- Dynamic regulation of Schwann cell enhancers after peripheral nerve injury.The Journal of biological chemistry · 2015Article
- Knockout of TLR4 and TLR2 impair the nerve regeneration by delayed demyelination but not remyelination.Journal of biomedical science · 2013Article
- Schwann cell myelination requires Dynein function.Neural development · 2012Article
- Live imaging of targeted cell ablation in Xenopus: a new model to study demyelination and repair.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Genome-wide analysis of EGR2/SOX10 binding in myelinating peripheral nerve.Nucleic acids research · 2012Article
- Distal enhancers upstream of the Charcot-Marie-Tooth type 1A disease gene PMP22.Human molecular genetics · 2012Article
- Targeted insertion of two Mthfr promoters in mice reveals temporal- and tissue-specific regulation.Mammalian genome : official journal of the International Mammalian Genome Society · 2011Article
- Towards resolving the transcription factor network controlling myelin gene expression.Nucleic acids research · 2011Article
- Regulatory modules function in a non-autonomous manner to control transcription of the mbp gene.Nucleic acids research · 2011Article
- Regulation of the PMP22 gene through an intronic enhancer.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Locus-wide identification of Egr2/Krox20 regulatory targets in myelin genes.Journal of neurochemistry · 2010Article
- Integrin-mediated axoglial interactions initiate myelination in the central nervous system.The Journal of cell biology · 2009Article
- Characterization of the regulatory region of the dopa decarboxylase gene in Medaka: an in vivo green fluorescent protein reporter assay combined with a simple TA-cloning method.Molecular biotechnology · 2009Article
- Review
- Review
- Separate proteolipid protein/DM20 enhancers serve different lineages and stages of development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myelin basic protein (MBP) gene expression is conferred in oligodendrocytes and Schwann cells by different upstream enhancers. In Schwann cells, expression is controlled by a 422 bp enhancer lying -9 kb from the gene. We show here that it contains 22 mammalian conserved motifs > or =6 bp. To investigate their functional significance, different combinations of wild-type or mutated motifs were introduced into reporter constructs that were inserted in single copy at a common hypoxanthine phosphoribosyltransferase docking site in embryonic stem cells. Lines of transgenic mice were derived, and the subsequent qualitative and quantitative expression phenotypes were compared at different stages of maturation. In the enhancer core, seven contiguous motifs cooperate to confer Schwann cell specificity while different combinations of flanking motifs engage, at different stages of Schwann cell maturation, to modulate expression level. Mutation of a Krox-20 binding site reduces the level of reporter expression, whereas mutation of a potential Sox element silences reporter expression. This potential Sox motif was also found conserved in other Schwann cell enhancers, suggesting that it contributes widely to regulatory function. These results demonstrate a close relationship between phylogenetic footprints and regulatory function and suggest a general model of enhancer organization. Finally, this investigation demonstrates that in vivo functional analysis, supported by controlled transgenesis, can be a robust complement to molecular and bioinformatics approaches to regulatory mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.