ArticleMolecular biology of the cell2006
Regulation of N-cadherin dynamics at neuronal contacts by ligand binding and cytoskeletal coupling.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- Electroactive Materials Surface Charge Impacts Neuron Viability and Maturation in 2D Cultures.ACS applied materials & interfaces · 2023Article
- Cell-cell interfaces as specialized compartments directing cell function.Nature reviews. Molecular cell biology · 2020Review
- Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.eLife · 2020Article
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- Spatial and temporal organization of cadherin in punctate adherens junctions.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Force-dependent binding of vinculin to α-catenin regulates cell-cell contact stability and collective cell behavior.Molecular biology of the cell · 2018Article
- A phenomenological model of cell-cell adhesion mediated by cadherins.Journal of mathematical biology · 2017Article
- Integration of Cadherin Adhesion and Cytoskeleton atCold Spring Harbor perspectives in biology · 2017Review
- Epithelial self-healing is recapitulated by a 3D biomimetic E-cadherin junction.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Computational Model for Kinetic Studies of Cadherin Binding and Clustering.Biophysical journal · 2016Article
- Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin.Nature communications · 2016Article
- There are four dynamically and functionally distinct populations of E-cadherin in cell junctions.Biology open · 2015Article
- The insulin response integrates increased TGF-β signaling through Akt-induced enhancement of cell surface delivery of TGF-β receptors.Science signaling · 2015Article
- The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity.The Journal of cell biology · 2015Article
- Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.Molecular biology of the cell · 2015Article
- GRIP1 interlinks N-cadherin and AMPA receptors at vesicles to promote combined cargo transport into dendrites.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N-cadherin plays a key role in axonal outgrowth and synaptogenesis, but how neurons initiate and remodel N-cadherin-based adhesions remains unclear. We addressed this issue with a semiartificial system consisting of N-cadherin coated microspheres adhering to cultured neurons transfected for N-cadherin-GFP. Using optical tweezers, we show that growth cones are particularly reactive to N-cadherin coated microspheres, which they capture in a few seconds and drag rearward. Such strong coupling requires an intact connection between N-cadherin receptors and catenins. As they move to the basis of growth cones, microspheres slow down while gradually accumulating N-cadherin-GFP, demonstrating a clear delay between bead coupling to the actin flow and receptor recruitment. Using FRAP and photoactivation, N-cadherin receptors at bead-to-cell contacts were found to continuously recycle, consistently with a model of ligand-receptor reaction not limited by membrane diffusion. The use of N-cadherin-GFP receptors truncated or mutated in specific cytoplasmic regions show that N-cadherin turnover is exquisitely regulated by catenin partners. Turnover rates are considerably lower than those obtained previously in single molecule studies, demonstrating an active regulation of cadherin bond kinetics in intact cells. Finally, spontaneous neuronal contacts enriched in N-cadherin exhibited similar turnover rates, suggesting that such dynamics of N-cadherin may represent an intrinsic mechanism underlying the plasticity of neuronal adhesions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.