ArticleMolecular biology of the cell2006
Protease nexin-1 promotes secretory granule biogenesis by preventing granule protein degradation.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
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- Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic β-cells.Nature genetics · 2018Article
- Chromogranins: from discovery to current times.Pflugers Archiv : European journal of physiology · 2018Review
- The surging role of Chromogranin A in cardiovascular homeostasis.Frontiers in chemistry · 2014Review
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Serpinins: role in granule biogenesis, inhibition of cell death and cardiac function.Current medicinal chemistry · 2012Article
- The extended granin family: structure, function, and biomedical implications.Endocrine reviews · 2011Review
- Role of pGlu-serpinin, a novel chromogranin A-derived peptide in inhibition of cell death.Journal of molecular neuroscience : MN · 2011Article
- Serpinin: a novel chromogranin A-derived, secreted peptide up-regulates protease nexin-1 expression and granule biogenesis in endocrine cells.Molecular endocrinology (Baltimore, Md.) · 2011Article
- HID-1, a new component of the peptidergic signaling pathway.Genetics · 2011Article
- Reprint of: Chromogranin A: a new proposal for trafficking, processing and induction of granule biogenesis.Regulatory peptides · 2010Article
- Pro-hormone secretogranin II regulates dense core secretory granule biogenesis in catecholaminergic cells.The Journal of biological chemistry · 2010Article
- Chromogranin A: a new proposal for trafficking, processing and induction of granule biogenesis.Regulatory peptides · 2010Review
- Carboxypeptidase E, an essential element of the regulated secretory pathway, is expressed and partially co-localized with chromogranin A in chicken thymus.Cell and tissue research · 2009Article
- Chromogranin A promotes peptide hormone sorting to mobile granules in constitutively and regulated secreting cells: role of conserved N- and C-terminal peptides.The Journal of biological chemistry · 2009Article
- Secretory trafficking signal encoded in the carboxyl-terminal region of the CGbeta-subunit.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Biogenesis and transport of secretory granules to release site in neuroendocrine cells.Journal of molecular neuroscience : MN · 2009Review
- Aquaporin 1 is important for maintaining secretory granule biogenesis in endocrine cells.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Sorting of the neuroendocrine secretory protein Secretogranin II into the regulated secretory pathway: role of N- and C-terminal alpha-helical domains.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dense-core secretory granule (DCG) biogenesis is a prerequisite step for the sorting, processing, and secretion of neuropeptides and hormones in (neuro)endocrine cells. Previously, chromogranin A (CgA) has been shown to play a key role in the regulation of DCG biogenesis in vitro and in vivo. However, the underlying mechanism of CgA-mediated DCG biogenesis has not been explored. In this study, we have uncovered a novel mechanism for the regulation of CgA-mediated DCG biogenesis. Transfection of CgA into endocrine 6T3 cells lacking CgA and DCGs not only recovered DCG formation and regulated secretion but also prevented granule protein degradation. Genetic profiling of CgA-expressing 6T3 versus CgA- and DCG-deficient 6T3 cells, followed by real-time reverse transcription-polymerase chain reaction and Western blotting analyses, revealed that a serine protease inhibitor, protease nexin-1 (PN-1), was significantly up-regulated in CgA-expressing 6T3 cells. Overexpression of PN-1 in CgA-deficient 6T3 cells prevented degradation of DCG proteins at the Golgi apparatus, enhanced DCG biogenesis, and recovered regulated secretion. Moreover, depletion of PN-1 by antisense RNAs in CgA-expressing 6T3 cells resulted in the specific degradation of DCG proteins. We conclude that CgA increases DCG biogenesis in endocrine cells by up-regulating PN-1 expression to stabilize granule proteins against degradation.
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