ArticleMolecular and cellular biochemistry2006
Evaluation of copper toxicity in isolated human peripheral blood mononuclear cells and it's attenuation by zinc: ex vivo.
Article in Molecular and cellular biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- Synergistic Angio-Osteogenic Effects of Copper-Releasing 3D Biocomposite Scaffolds: A Step Toward Vascularized Bone Regeneration.Research square · 2025Article
- Essential and Non-Essential Metals and Metalloids and Their Role in Atherosclerosis.Cardiovascular toxicology · 2025Review
- Immunomodulatory Effects of Copper Bis-Glycinate In Vitro.Molecules (Basel, Switzerland) · 2025Article
- Deciphering the Role of Copper Homeostasis in Atherosclerosis: From Molecular Mechanisms to Therapeutic Targets.International journal of molecular sciences · 2024Review
- Feasibility study on Ti-15Mo-7Cu with low elastic modulus and high antibacterial property.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Article
- In Vitro Supplementation of Copper Modulates the Functional Th1/Th2 Phenotype of Peripheral Blood Mononuclear Cells in Cattle.Animals : an open access journal from MDPI · 2021Article
- Copper-based biomaterials for bone and cartilage tissue engineering.Journal of orthopaedic translation · 2021Review
- In vitro and in vivo effects of flubendiamide and copper on cyto-genotoxicity, oxidative stress and spleen histology of rats and its modulation by resveratrol, catechin, curcumin and α-tocopherol.BMC pharmacology & toxicology · 2020Article
- High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells.Environmental science and pollution research international · 2017Article
- Transition metals activate TFEB in overexpressing cells.The Biochemical journal · 2015Article
- Biocorrosion properties of antibacterial Ti-10Cu sintered alloy in several simulated biological solutions.Journal of materials science. Materials in medicine · 2015Article
- Labelling of granulocytes by phagocytic engulfment with 64Cu-labelled chitosan-coated magnetic nanoparticles.Molecular imaging and biology · 2012Article
- Copper accelerates glycolytic flux in cultured astrocytes.Neurochemical research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper and zinc act as a cofactor of over 300 mammalian proteins. Both have same electronic configuration therefore they are antagonist at higher individual concentration. The present study was designed with the aim to investigate the mechanisms pertaining to toxic effects of copper on human peripheral blood mononuclear cells (PBMCs) and to evaluate the cytoprotective effect of zinc on copper-induced cytotoxicity. The copper uptake into PBMCs was progressively increased with increasing concentration of metal in the growth medium. However, no significant effect on copper uptake was observed in the presence of zinc. Cell proliferation rate was decreased with increasing copper concentration. Interestingly, the proliferation rate of zinc treated PBMCs remained nearly the same as that of control cells. LD(50) of copper (115 microM) was increased six times (710 microM) in presence of zinc for PBMCs. At higher concentrations of copper (> 100 microM) decrease level of GSH was noticed. Increased levels of metallothionein in PBMCs were observed in response to zinc. DNA fragmentation studies also showed that copper produced DNA fragmentation at LD(50) (115 microM). Subsequently, zinc showed protection against DNA fragmentation caused by copper. Cell structure of PBMCs at LD(50) (115 microM copper) showed membrane bound cystic spaces and mitochondria having disrupted cristae and few myelin figures. In presence of zinc at LD(50) of copper (115 microM) cells showed improvement in mitochondrial structure and membrane bound cystic spaces. Taken together, the results of our study demonstrates that zinc play an important role in prevention of copper toxicity in peripheral blood mononuclear cells.
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