ArticleLangmuir : the ACS journal of surfaces and colloids2005
E-cadherin tethered to micropatterned supported lipid bilayers as a model for cell adhesion.
Article in Langmuir : the ACS journal of surfaces and colloids, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Mimicking Cellular Membranes With Micropatterned Lipid Bilayers.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Dimensional Reduction for Single-Molecule Imaging of DNA and Nucleosome Condensation by Polyamines, HP1α and Ki-67.The journal of physical chemistry. B · 2023Article
- Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes.Journal of the American Chemical Society · 2021Article
- Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications.Polymers · 2020Review
- Supported lipid bilayer platforms to probe cell mechanobiology.Biochimica et biophysica acta. Biomembranes · 2017Review
- E-cadherin junction formation involves an active kinetic nucleation process.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Membrane adhesion and the formation of heterogeneities: biology, biophysics, and biotechnology.Physical chemistry chemical physics : PCCP · 2015Review
- Force measurement tools to explore cadherin mechanotransduction.Cell communication & adhesion · 2014Review
- Micropatterned, multicomponent supported lipid bilayers for cellular systems.Methods in cell biology · 2014Article
- Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides.Biomaterials · 2010Article
- Lateral Mobility of E-cadherin Enhances Rac1 Response in Epithelial Cells.Cellular and molecular bioengineering · 2010Article
- Nanofabricated racks of aligned and anchored DNA substrates for single-molecule imaging.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Capturing the nanoscale complexity of cellular membranes in supported lipid bilayers.Journal of structural biology · 2009Review
- Self-aligned supported lipid bilayers for patterning the cell-substrate interface.Journal of the American Chemical Society · 2009Article
- Stable, ligand-doped, poly(bis-SorbPC) lipid bilayer arrays for protein binding and detection.ACS applied materials & interfaces · 2009Article
- Rigidity-dependent cross talk between integrin and cadherin signaling.Biophysical journal · 2009Article
- Effects of supported lipid monolayer fluidity on the adhesion of hematopoietic progenitor cell lines to fibronectin-derived peptide ligands for alpha5beta1 and alpha4beta1 integrins.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Diffusion in supported lipid bilayers: influence of substrate and preparation technique on the internal dynamics.The European physical journal. E, Soft matter · 2009Article
- Nanoengineering of Immune Cell Function.Materials Research Society symposia proceedings. Materials Research Society · 2009Article
- Micropatterned fluid lipid bilayer arrays created using a continuous flow microspotter.Analytical chemistry · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cell-cell adhesion is a dynamic process requiring recruitment, binding, and reorganization of signaling proteins in the plane of the plasma membrane. Here, we describe a new system for investigating how this lateral mobility influences cadherin-based cell signaling. This model is based on tethering of a GPI-modified E-cadherin protein (hEFG) to a supported lipid bilayer. In this report, membrane microfluidics and micropatterning techniques are used to adopt this tethered protein system for studies with the anchorage-dependent cells. As directly formed from proteoliposomes, hEFG exhibits a diffusion coefficient of 0.6 +/- 0.3 microm(2)/s and mobile fraction of 30-60%. Lateral structuring of the supported lipid bilayer is used to isolate mobile proteins from this mixed mobile/immobile population, and should be widely applicable to other proteins. MCF-7 cells seeded onto hEFG-containing bilayers recognize and cluster this protein, but do not exhibit cell spreading required for survival. By micropatterning small anchors into the supported lipid bilayer, we have achieved cell spreading across the bilayer surface and concurrent interaction with mobile hEFG protein. Together, these techniques will allow more detailed analysis of the cellular dynamics involved in cadherin-dependent adhesion events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.