ArticleMolecular and cellular biology2005
Evidence for high-capacity bidirectional glucose transport across the endoplasmic reticulum membrane by genetically encoded fluorescence resonance energy transfer nanosensors.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 85 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- Resolving subcellular sucrose concentrations in plant tissues.Journal of experimental botany · 2026Review
- Evidence for rapid hydrolysis of shoot-derived sucrose using an ultrasensitive ratiometric matryoshka-type MGlucoMeter sensor.The Plant journal : for cell and molecular biology · 2026Article
- iGlucoSnFR2: A genetically encoded fluorescent sensor for measuring intracellular or extracellular glucose in vivo in mouse brain.Science advances · 2025Article
- Dynamic glucose uptake, storage, and release by human microvascular endothelial cells.Molecular biology of the cell · 2022Article
- Luseogliflozin, a SGLT2 Inhibitor, Does Not Affect Glucose Uptake Kinetics in Renal Proximal Tubules of Live Mice.International journal of molecular sciences · 2021Article
- An alternative pathway for sweet sensation: possible mechanisms and physiological relevance.Pflugers Archiv : European journal of physiology · 2020Review
- Glucose Transport and Transporters in the Endomembranes.International journal of molecular sciences · 2019Review
- Effective Glucose Uptake by Human Astrocytes Requires Its Sequestration in the Endoplasmic Reticulum by Glucose-6-Phosphatase-β.Current biology : CB · 2018Article
- Glucose Transporters and Virulence inmSphere · 2018Article
- Occludin regulates glucose uptake and ATP production in pericytes by influencing AMP-activated protein kinase activity.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
- A novel probe based on phenylboronic acid functionalized carbon nanotubes for ultrasensitive carbohydrate determination in biofluids and semi-solid biotissues.Chemical science · 2016Article
- Article
- Mitoenergetic Dysfunction Triggers a Rapid Compensatory Increase in Steady-State Glucose Flux.Biophysical journal · 2015Article
- Glucose transport machinery reconstituted in cell models.Chemical communications (Cambridge, England) · 2015Article
- Single-cell imaging tools for brain energy metabolism: a review.Neurophotonics · 2014Article
- FIBS-enabled noninvasive metabolic profiling.Journal of visualized experiments : JoVE · 2014Article
- High glucose potentiates L-FABP mediated fibrate induction of PPARα in mouse hepatocytes.Biochimica et biophysica acta · 2013Article
- Impact of L-FABP and glucose on polyunsaturated fatty acid induction of PPARα-regulated β-oxidative enzymes.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glucose release from hepatocytes is important for maintenance of blood glucose levels. Glucose-6-phosphate phosphatase, catalyzing the final metabolic step of gluconeogenesis, faces the endoplasmic reticulum (ER) lumen. Thus, glucose produced in the ER has to be either exported from the ER into the cytosol before release into circulation or exported directly by a vesicular pathway. To measure ER transport of glucose, fluorescence resonance energy transfer-based nanosensors were targeted to the cytosol or the ER lumen of HepG2 cells. During perfusion with 5 mM glucose, cytosolic levels were maintained at approximately 80% of the external supply, indicating that plasma membrane transport exceeded the rate of glucose phosphorylation. Glucose levels and kinetics inside the ER were indistinguishable from cytosolic levels, suggesting rapid bidirectional glucose transport across the ER membrane. A dynamic model incorporating rapid bidirectional ER transport yields a very good fit with the observed kinetics. Plasma membrane and ER membrane glucose transport differed regarding sensitivity to cytochalasin B and showed different relative kinetics for galactose uptake and release, suggesting catalysis by distinct activities at the two membranes. The presence of a high-capacity glucose transport system on the ER membrane is consistent with the hypothesis that glucose export from hepatocytes occurs via the cytosol by a yet-to-be-identified set of proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.