ArticleMolecular and cellular biology2005
Identification of functionally important amino acids of ribosomal protein L3 by saturation mutagenesis.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 58 citations in OpenAlex.
- Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart.Nucleic acids research · 2023Article
- RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function.Nature communications · 2023Article
- Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing.Viruses · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- A functional connection between translation elongation and protein folding at the ribosome exit tunnel in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- NEP-TC a rRNA Methyltransferase Involved on Somatic Embryogenesis of Tamarillo (Frontiers in plant science · 2019Article
- Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth.Journal of cellular physiology · 2016Article
- Ribosomal Antibiotics: Contemporary Challenges.Antibiotics (Basel, Switzerland) · 2016Review
- Crystal Structures of the uL3 Mutant Ribosome: Illustration of the Importance of Ribosomal Proteins for Translation Efficiency.Journal of molecular biology · 2016Article
- Methylation of yeast ribosomal protein Rpl3 promotes translational elongation fidelity.RNA (New York, N.Y.) · 2016Article
- Bypass of the pre-60S ribosomal quality control as a pathway to oncogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Eukaryotic rpL10 drives ribosomal rotation.Nucleic acids research · 2014Article
- Preparation of biologically active Arabidopsis ribosomes and comparison with yeast ribosomes for binding to a tRNA-mimic that enhances translation of plant plus-strand RNA viruses.Frontiers in plant science · 2013Article
- The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress.BMC molecular biology · 2012Article
- Current and future experimental strategies for structural analysis of trichothecene mycotoxins--a prospectus.Toxins · 2011Review
- Chromatographic purification of highly active yeast ribosomes.Journal of visualized experiments : JoVE · 2011Article
- Evolution of a helper virus-derived, ribosome binding translational enhancer in an untranslated satellite RNA of Turnip crinkle virus.Virology · 2011Article
- Article
- Ribosome binding to a 5' translational enhancer is altered in the presence of the 3' untranslated region in cap-independent translation of turnip crinkle virus.Journal of virology · 2011Article
- Endogenous ribosomal frameshift signals operate as mRNA destabilizing elements through at least two molecular pathways in yeast.Nucleic acids research · 2011Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
There is accumulating evidence that many ribosomal proteins are involved in shaping rRNA into their functionally correct conformations through RNA-protein interactions. Moreover, although rRNA seems to play the central role in all aspects of ribosome function, ribosomal proteins may be involved in facilitating communication between different functional regions in ribosome, as well as between the ribosome and cellular factors. In an effort to more fully understand how ribosomal proteins may influence ribosome function, we undertook large-scale mutational analysis of ribosomal protein L3, a core protein of the large subunit that has been implicated in numerous ribosome-associated functions in the past. A total of 98 different rpl3 alleles were genetically characterized with regard to their effects on killer virus maintenance, programmed -1 ribosomal frameshifting, resistance/hypersensitivity to the translational inhibitor anisomycin and, in specific cases, the ability to enhance translation of a reporter mRNA lacking the 5' (7)mGppp cap structure and 3' poly(A) tail. Biochemical studies reveal a correlation between an increased affinity for aminoacyl-tRNA and the extent of anisomycin resistance and a decreased peptidyltransferase activity and increased frameshifting efficiency. Immunoblot analyses reveal that the superkiller phenotype is not due to a defect in the ability of ribosomes to recruit the Ski-complex, suggesting that the defect lies in a reduced ability of mutant ribosomes to distinguish between cap(+)/poly(A)(+) and cap(-)/poly(A)(-) mRNAs. The results of these analyses are discussed with regard to how protein-rRNA interactions may affect ribosome function.
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