ArticleThe American journal of pathology2005
Biomimetic delivery of keratinocyte growth factor upon cellular demand for accelerated wound healing in vitro and in vivo.
Article in The American journal of pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 118 citations in OpenAlex.
- Increased thermal stability of FGF10 leads to ectopic signaling during development.Cellular and molecular life sciences : CMLS · 2025Article
- Fibrin hydrogels fortified with FGF-7/10 and laminin-1 peptides promote regeneration of irradiated salivary glands.Acta biomaterialia · 2023Article
- Novel strategies for designing regenerative skin products for accelerated wound healing.3 Biotech · 2022Review
- Growth Factor and Cytokine Delivery Systems for Wound Healing.Cold Spring Harbor perspectives in biology · 2022Review
- Fibroblast Growth Factor 7 Suppresses Fibrosis and Promotes Epithelialization during Wound Healing in Mouse Fetuses.International journal of molecular sciences · 2022Article
- Engineering Extracellular Microenvironment for Tissue Regeneration.Bioengineering (Basel, Switzerland) · 2022Review
- Engineered Molecular Therapeutics Targeting Fibrin and the Coagulation System: a Biophysical Perspective.Biophysical reviews · 2022Review
- Trimers Conjugated to Fibrin Hydrogels Promote Salivary Gland Function.Journal of dental research · 2021Article
- Biomimetic hydrogels with spatial- and temporal-controlled chemical cues for tissue engineering.Biomaterials science · 2020Review
- Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels.Acta biomaterialia · 2020Article
- Systemically Administered, Target-Specific, Multi-Functional Therapeutic Recombinant Proteins in Regenerative Medicine.Nanomaterials (Basel, Switzerland) · 2020Review
- Synergistic effects of laminin-1 peptides, VEGF and FGF9 on salivary gland regeneration.Acta biomaterialia · 2019Article
- Skin tissue regeneration for burn injury.Stem cell research & therapy · 2019Review
- Bioactivity Determination of a Therapeutic Recombinant Human Keratinocyte Growth Factor by a Validated Cell-based Bioassay.Molecules (Basel, Switzerland) · 2019Article
- Drug delivery and tissue engineering to promote wound healing in the immunocompromised host: Current challenges and future directions.Advanced drug delivery reviews · 2018Review
- ECM turnover-stimulated gene delivery through collagen-mimetic peptide-plasmid integration in collagen.Acta biomaterialia · 2017Article
- FOXO1 expression in keratinocytes promotes connective tissue healing.Scientific reports · 2017Article
- Laminin-111 Peptides Conjugated to Fibrin Hydrogels Promote Formation of Lumen Containing Parotid Gland Cell Clusters.Biomacromolecules · 2016Article
- Biomimetic scaffolds for regeneration of volumetric muscle loss in skeletal muscle injuries.Acta biomaterialia · 2015Review
- Systemically Administered, Target Organ-Specific Therapies for Regenerative Medicine.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Exogenous keratinocyte growth factor (KGF) significantly enhances wound healing, but its use is hampered by a short biological half-life and lack of tissue selectivity. We used a biomimetic approach to achieve cell-controlled delivery of KGF by covalently attaching a fluorescent matrix-binding peptide that contained two domains: one recognized by factor XIII and the other by plasmin. Modified KGF was incorporated into the fibrin matrix at high concentration in a factor XIII-dependent manner. Cell-mediated activation of plasminogen to plasmin degraded the fibrin matrix and cleaved the peptides, releasing active KGF to the local microenvironment and enhancing epithelial cell proliferation and migration. To demonstrate in vivo effectiveness, we used a hybrid model of wound healing that involved transplanting human bioengineered skin onto athymic mice. At 6 weeks after grafting, the transplanted tissues underwent full thickness wounding and treatment with fibrin gels containing bound KGF. In contrast to topical KGF, fibrin-bound KGF persisted in the wounds for several days and was released gradually, resulting in significantly enhanced wound closure. A fibrinolytic inhibitor prevented this healing, indicating the requirement for cell-mediated fibrin degradation to release KGF. In conclusion, this biomimetic approach of localized, cell-controlled delivery of growth factors may accelerate healing of large full-thickness wounds and chronic wounds that are notoriously difficult to heal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.