ArticleMolecular biology of the cell2006
Biogenesis of tubular ER-to-Golgi transport intermediates.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Plant Golgi and GECCO: The Universality of ER-Golgi Interface.Sub-cellular biochemistry · 2026Review
- The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes.Molecular biology of the cell · 2024Article
- Rab6-mediated retrograde trafficking from the Golgi: the trouble with tubules.Small GTPases · 2023Review
- Membrane Trafficking Mechanisms and Their Biological Relevance.Archives of Razi Institute · 2023Review
- Regulation of the COPII secretory machinery via focal adhesions and extracellular matrix signaling.The Journal of cell biology · 2022Article
- SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.Developmental cell · 2022Article
- Article
- Review
- Biological lipid nanotubes and their potential role in evolution.The European physical journal. Special topics · 2020Review
- Differentiation of Trafficking Pathways at Golgi Entry Core Compartments and Post-Golgi Subdomains.Frontiers in plant science · 2020Review
- Article
- Intermediate compartment (IC): from pre-Golgi vacuoles to a semi-autonomous membrane system.Histochemistry and cell biology · 2018Review
- p24 family proteins: key players in the regulation of trafficking along the secretory pathway.Protoplasma · 2016Review
- Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport.Biology open · 2014Article
- Golgi tubules: their structure, formation and role in intra-Golgi transport.Histochemistry and cell biology · 2013Review
- Glycosyltransferase-specific Golgi-targeting mechanisms.The Journal of biological chemistry · 2012Article
- Golgin160 recruits the dynein motor to position the Golgi apparatus.Developmental cell · 2012Article
- Coupled transport of Arabidopsis p24 proteins at the ER-Golgi interface.Journal of experimental botany · 2012Article
- ADP ribosylation factors 1 and 4 and group VIA phospholipase A₂ regulate morphology and intraorganellar traffic in the endoplasmic reticulum-Golgi intermediate compartment.Molecular biology of the cell · 2010Article
- Contributions of electron microscopy to understand secretion of immune mediators by human eosinophils.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tubular transport intermediates (TTIs) have been described as one class of transport carriers in endoplasmic reticulum (ER)-to-Golgi transport. In contrast to vesicle budding and fusion, little is known about the molecular regulation of TTI synthesis, transport and fusion with target membranes. Here we have used in vivo imaging of various kinds of GFP-tagged proteins to start to address these questions. We demonstrate that under steady-state conditions TTIs represent approximately 20% of all moving transport carriers. They increase in number and length when more transport cargo becomes available at the donor membrane, which we induced by either temperature-related transport blocks or increased expression of the respective GFP-tagged transport markers. The formation and motility of TTIs is strongly dependent on the presence of intact microtubules. Microinjection of GTPgammaS increases the frequency of TTI synthesis and the length of these carriers. When Rab proteins are removed from membranes by microinjection of recombinant Rab-GDI, the synthesis of TTIs is completely blocked. Microinjection of the cytoplasmic tails of the p23 and p24 membrane proteins also abolishes formation of p24-containing TTIs. Our data suggest that TTIs are ER-to-Golgi transport intermediates that form preferentially when transport-competent cargo exists in excess at the donor membrane. We propose a model where the interaction of the cytoplasmic tails of membrane proteins with microtubules are key determinants for TTI synthesis and may also serve as a so far unappreciated model for aspects of transport carrier formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.