ReviewNucleic acids research2005
A second look at cellular mRNA sequences said to function as internal ribosome entry sites.
Review in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 165 citations in OpenAlex.
- A B1 SINE element within the mouseScience advances · 2026Article
- Reassessment of IRES Element Activity inInternational journal of molecular sciences · 2026Article
- Translational and epitranscriptomic regulation of seed germination in Arabidopsis thaliana genotypes with contrasting dormancy phenotypes.Plant molecular biology · 2025Article
- The patterns of alternative TSS usage explain the highly heterogeneous landscape of 5'UTR lengths in eukaryotes.NAR genomics and bioinformatics · 2025Article
- Enhancing cap-independent translation of linear mRNA.Nature communications · 2025Article
- Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR).Nature structural & molecular biology · 2025Review
- A Review on Circular RNA Translation and Its Implications in Disease.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Emerging roles and potential clinical applications of translatable circular RNAs in cancer and other human diseases.Genes & diseases · 2023Review
- False-positive IRESes fromProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Pervasive translation of circular RNAs driven by short IRES-like elements.Nature communications · 2022Article
- Reversal of G-Quadruplexes' Role in Translation Control When Present in the Context of an IRES.Biomolecules · 2022Review
- Skin-specific transgenic overexpression of ovine β-catenin in mice.Frontiers in genetics · 2022Article
- Translation initiation and its relevance in colorectal cancer.The FEBS journal · 2021Review
- Differential bicistronic gene translation mediated by the internal ribosome entry site element of encephalomyocarditis virus.Biomedical journal · 2021Article
- AMPK-mTOR Signaling and Cellular Adaptations in Hypoxia.International journal of molecular sciences · 2021Review
- Article
- Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.Biochemistry. Biokhimiia · 2021Review
- Revisiting the pathogenic mechanism of the GJB1 5' UTR c.-103C > T mutation causing CMTX1.Neurogenetics · 2021Article
- Hypoxia/HIF Modulates Immune Responses.Biomedicines · 2021Review
- A novel transgenic mouse strain expressing PKCβII demonstrates expansion of B1 and marginal zone B cell populations.Scientific reports · 2020Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This review takes a second look at a set of mRNAs that purportedly employ an alternative mechanism of initiation when cap-dependent translation is reduced during mitosis or stress conditions. A closer look is necessary because evidence cited in support of the internal initiation hypothesis is often flawed. When putative internal ribosome entry sequences (IRESs) are examined more carefully, they often turn out to harbor cryptic promoters or splice sites. This undermines the dicistronic assay, wherein IRES activity is measured by the ability to support translation of the 3' cistron. Most putative IRESs still have not been checked carefully to determine whether the dicistronic vector produces only the intended dicistronic mRNA. The widespread use of the pRF vector is a major problem because this vector, which has Renilla luciferase as the 5' cistron and firefly luciferase as the 3' cistron, has been found to generate spliced transcripts. RNA transfection assays could theoretically circumvent these problems, but most candidate IRESs score very weakly in that test. The practice of calling even very weak results 'positive' is one of the problems discussed herein. The extremely low efficiency of putative IRESs is inconsistent with their postulated biological roles.'
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.