ArticleNucleic acids research2005
Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
- Limited effect of PCR ramp rate on qPCR Ct values: an exploratory study.Molecular biology reports · 2026Article
- Viral Load Measurements for Kaposi Sarcoma Herpesvirus (KSHV/HHV8): Review and an Updated Assay.Journal of medical virology · 2024Review
- FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.International journal of molecular sciences · 2024Article
- Pediatric HIV+ Kaposi sarcoma exhibits clinical, virological, and molecular features different from the adult disease.JCI insight · 2023Article
- Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective.PloS one · 2022Article
- Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.Pathogens (Basel, Switzerland) · 2021Article
- High-Density Amplicon Sequencing Identifies Community Spread and Ongoing Evolution of SARS-CoV-2 in the Southern United States.Cell reports · 2020Article
- Runaway Kaposi Sarcoma-associated herpesvirus replication correlates with systemic IL-10 levels.Virology · 2020Article
- Mutant Cellular AP-1 Proteins Promote Expression of a Subset of Epstein-Barr Virus Late Genes in the Absence of Lytic Viral DNA Replication.Journal of virology · 2018Article
- Data supporting the functional role of Eleven-nineteen Lysine-rich Leukemia 3 (ELL3) in B cell lymphoma cell line cells.Data in brief · 2017Article
- qPCR primer design revisited.Biomolecular detection and quantification · 2017Review
- Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.Molecular immunology · 2017Article
- Plasma Epstein-Barr virus DNA for pediatric Burkitt lymphoma diagnosis, prognosis and response assessment in Malawi.International journal of cancer · 2017Article
- Article
- Systems biologic analysis of T regulatory cells genetic pathways in murine primary biliary cirrhosis.Journal of autoimmunity · 2015Article
- Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance.Frontiers in microbiology · 2014Article
- Gene number determination and genetic polymorphism of the gamma delta T cell co-receptor WC1 genes.BMC genetics · 2012Article
- Shutoff of BZLF1 gene expression is necessary for immortalization of primary B cells by Epstein-Barr virus.Journal of virology · 2012Article
- SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR.BMC research notes · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Quantitative real-time PCR has become the method of choice for measuring mRNA transcription. Recently, fast PCR protocols have been developed as a means to increase assay throughput. Yet it is unclear whether more rapid cycling conditions preserve the original assay performance characteristics. We compared 16 primer sets directed against Epstein-Barr virus (EBV) mRNAs using universal and fast PCR cycling conditions. These primers are of clinical relevance, since they can be used to monitor viral oncogene and drug-resistance gene expression in transplant patients and EBV-associated cancers. While none of the primers failed under fast PCR conditions, the fast PCR protocols performed worse than universal cycling conditions. Fast PCR was associated with a loss of sensitivity as well as higher variability, but not with a loss of specificity or with a higher false positive rate.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.