ArticleProceedings of the National Academy of Sciences of the United States of America1992
Localized torsional tension in the DNA of human cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 96 citations in OpenAlex.
- Topoisomerase-modulated genome-wide DNA supercoiling domains colocalize with nuclear compartments and regulate human gene expression.Nature structural & molecular biology · 2025Article
- Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters.Science advances · 2023Article
- Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters.bioRxiv : the preprint server for biology · 2023Article
- Supercoiling and looping promote DNA base accessibility and coordination among distant sites.Nature communications · 2021Article
- Transcription-mediated supercoiling regulates genome folding and loop formation.Molecular cell · 2021Article
- Superresolution imaging of chromatin fibers to visualize epigenetic information on replicative DNA.Nature protocols · 2020Article
- Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement.Nature structural & molecular biology · 2019Article
- Are TADs supercoiled?Nucleic acids research · 2019Review
- Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint.PloS one · 2019Article
- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Investigating DNA supercoiling in eukaryotic genomes.Briefings in functional genomics · 2017Review
- Genome urbanization: clusters of topologically co-regulated genes delineate functional compartments in the genome of Saccharomyces cerevisiae.Nucleic acids research · 2017Article
- Genome scale patterns of supercoiling in a bacterial chromosome.Nature communications · 2016Article
- On the role of inter-nucleosomal interactions and intrinsic nucleosome dynamics in chromatin function.Biochemistry and biophysics reports · 2016Review
- Effects of DNA supercoiling on chromatin architecture.Biophysical reviews · 2016Review
- Effects of DNA supercoiling on chromatin architecture.Biophysical reviews · 2016Review
- Profiling DNA supercoiling domains in vivo.Genomics data · 2014Article
- Rate of elongation by RNA polymerase II is associated with specific gene features and epigenetic modifications.Genome research · 2014Article
- Supercoiling in DNA and chromatin.Current opinion in genetics & development · 2014Review
- DNA topology in chromosomes: a quantitative survey and its physiological implications.Journal of mathematical biology · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Torsional tension in DNA may be both a prerequisite for the efficient initiation of transcription and a consequence of the transcription process itself with the generation of positive torsional tension in front of the RNA polymerase and negative torsional tension behind it. To examine torsional tension in specific regions of genomic DNA in vivo, we developed an assay using photoactivated psoralen as a probe for unconstrained DNA superhelicity and x-rays as a means to relax DNA. Psoralen intercalates more readily into DNA underwound by negative torsional tension than into relaxed. DNA, and it can form interstrand DNA cross-links upon UVA irradiation. By comparing the amount of psoralen-induced DNA cross-links in cells irradiated with x-rays either before or after the psoralen treatment, we examined the topological state of the DNA in specific regions of the genome in cultured human 6A3 cells. We found that although no net torsional tension was detected in the bulk of the genome, localized tension was prominent in the DNA of two active genes. Negative torsional tension was found in the 5' end of the amplified dihydrofolate reductase gene and in a region near the 5' end of the 45S rRNA transcription unit, whereas a low level of positive torsional tension was found in a region near the 3' end of the dihydrofolate reductase gene. These results document an intragenomic heterogeneity of DNA torsional tension and lend support to the twin supercoiled domain model for transcription in the genome of intact human cells.
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