ReviewCurrent genetics2006
RNA silencing in Chlamydomonas: mechanisms and tools.
Review in Current genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 140 citations in OpenAlex.
- Heterotrophic fermentation of a robust human defensin in Chlamydomonas reinhardtii provides a stable and potent antibacterial.Microbial cell factories · 2026Article
- A Blue Light-Responsive Strong Synthetic Promoter Based on Rational Design inInternational journal of molecular sciences · 2023Article
- Cloning and expression of an anti-cancerous cytokine: human IL-29 gene in Chlamydomonas reinhardtii.AMB Express · 2023Article
- Increased Lipids inInternational journal of molecular sciences · 2022Article
- A microalgal-based preparation with synergistic cellulolytic and detoxifying action towards chemical-treated lignocellulose.Plant biotechnology journal · 2021Article
- The small RNA locus map for Chlamydomonas reinhardtii.PloS one · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Biomass from microalgae: the potential of domestication towards sustainable biofactories.Microbial cell factories · 2018Review
- Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii.Journal of experimental botany · 2018Article
- Identification of Insertion Site by RESDA-PCR inBio-protocol · 2018Article
- Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.The Plant journal : for cell and molecular biology · 2017Article
- Building a multipurpose insertional mutant library for forward and reverse genetics inPlant methods · 2017Article
- RNAi in Plants: An Argonaute-Centered View.The Plant cell · 2016Review
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses.Photosynthesis research · 2015Review
- The Chlamydomonas genome project: a decade on.Trends in plant science · 2014Review
- Unraveling vitamin B12-responsive gene regulation in algae.Plant physiology · 2014Article
- Article
- funRNA: a fungi-centered genomics platform for genes encoding key components of RNAi.BMC genomics · 2014Article
- Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed at reducing target gene expression in Chlamydomonas via the stable expression of antisense or inverted repeat-containing RNA. Double-stranded RNA (dsRNA) generated by the annealing of antisense and sense RNAs or by hairpin formation of an inverted repeat, feeds into the RNA silencing pathway. In this pathway, dsRNA is cleaved into approximately 25-bp small interfering RNAs (siRNAs) by the endonuclease Dicer. One of the two complementary strands of a siRNA is then loaded onto an Argonaute-like protein present as core component within larger complexes. Guided by this single-stranded RNA, the Argonaute-like protein either detects homologous transcripts and cleaves these endonucleolytically, or initiates transcriptional gene silencing. This article summarizes current information derived mainly from the Chlamydomonas genome project on components that are assumed to be involved in RNA silencing mechanisms in Chlamydomonas. Furthermore, all approaches employed in Chlamydomonas to date to downregulate target gene expression by antisense or inverted repeat constructs are reviewed and discussed critically.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.