ArticleMolecular genetics and genomics : MGG2006
Constancy of organellar genome copy numbers during leaf development and senescence in higher plants.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- GIF1 controls ear inflorescence architecture and floral development by regulating key genes in hormone biosynthesis and meristem determinacy in maize.BMC plant biology · 2022Article
- Avoiding organelle mutational meltdown across eukaryotes with or without a germline bottleneck.PLoS biology · 2021Article
- A rapid and efficient method for enriching mitochondrial DNA from plants.Mitochondrial DNA. Part B, Resources · 2018Article
- Article
- Opposite roles of the Arabidopsis cytokinin receptors AHK2 and AHK3 in the expression of plastid genes and genes for the plastid transcriptional machinery during senescence.Plant molecular biology · 2017Article
- Organellar genome copy number variation and integrity during moderate maturation of roots and leaves of maize seedlings.Current genetics · 2015Article
- DNA maintenance in plastids and mitochondria of plants.Frontiers in plant science · 2015Review
- Changes in DNA damage, molecular integrity, and copy number for plastid DNA and mitochondrial DNA during maize development.Journal of experimental botany · 2014Article
- On the fate of plastid DNA molecules during leaf development: response to the Golczyk et al. Commentary.The Plant cell · 2014Article
- Chloroplast DNA in mature and senescing leaves: a reappraisal.The Plant cell · 2014Article
- Effects of reduced chloroplast gene copy number on chloroplast gene expression in maize.Plant physiology · 2012Article
- Distinguishing authentic mitochondrial and plastid DNAs from similar DNA sequences in the nucleus using the polymerase chain reaction.Current genetics · 2011Article
- A conserved, Mg²+-dependent exonuclease degrades organelle DNA during Arabidopsis pollen development.The Plant cell · 2011Article
- Dismantling of Arabidopsis thaliana mesophyll cell chloroplasts during natural leaf senescence.Plant biology (Stuttgart, Germany) · 2010Article
- Variable amounts of DNA related to the size of chloroplasts III. Biochemical determinations of DNA amounts per organelle.Molecular genetics and genomics : MGG · 2010Article
- Genome-wide analysis of plastid gene expression in potato leaf chloroplasts and tuber amyloplasts: transcriptional and posttranscriptional control.Plant physiology · 2009Article
- Extensive homologous recombination between introduced and native regulatory plastid DNA elements in transplastomic plants.Transgenic research · 2009Article
- A multiple-method approach reveals a declining amount of chloroplast DNA during development in Arabidopsis.BMC plant biology · 2009Article
- Coordination of gene expression between organellar and nuclear genomes.Nature reviews. Genetics · 2008Review
- The structure of chloroplast DNA molecules and the effects of light on the amount of chloroplast DNA during development in Medicago truncatula.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher plants, plastid and mitochondrial genomes occur at high copy numbers per cell. Several recent publications have suggested that, in higher plants like Arabidopsis and maize, chloroplast DNA is virtually absent in mature and old leaves. This conclusion was mainly based on DAPI staining of isolated chloroplasts. If correct, the finding that chloroplasts in mature leaves lack DNA would change dramatically our understanding of gene expression, mRNA stability and protein stability in chloroplasts. In view of the wide implications that the disposal of chloroplast DNA during leaf development would have, we have reinvestigated the age dependency of genome copy numbers in chloroplasts and, in addition, tested for possible changes in mitochondrial genome copy number during plant development. Analyzing chloroplast and mitochondrial DNA amounts in Arabidopsis and tobacco plants, we find that organellar genome copy numbers remain remarkably constant during leaf development and are present in essentially unchanged numbers even in the senescing leaves. We conclude that, during leaf development, organellar gene expression in higher plants is not significantly regulated at the level of genome copy number and we discuss possible explanations for the failure to detect DNA in isolated chloroplasts stained with DAPI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.