ArticleThe EMBO journal2005
P-TEFb is not an essential elongation factor for the intronless human U2 snRNA and histone H2b genes.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 83 citations in OpenAlex.
- Cajal bodies: Evolutionarily conserved nuclear biomolecular condensates with properties unique to plants.The Plant cell · 2023Review
- G4access identifies G-quadruplexes and their associations with open chromatin and imprinting control regions.Nature genetics · 2023Article
- An Important Role for RPRD1B in the Heat Shock Response.Molecular and cellular biology · 2022Article
- CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors.Biomolecules · 2022Article
- Live imaging of transcription sites using an elongating RNA polymerase II-specific probe.The Journal of cell biology · 2022Article
- Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination.Transcription · 2021Article
- RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells.Genes to cells : devoted to molecular & cellular mechanisms · 2021Article
- CDK11 is required for transcription of replication-dependent histone genes.Nature structural & molecular biology · 2020Article
- 7SK small nuclear RNA, a multifunctional transcriptional regulatory RNA with gene-specific features.Transcription · 2018Article
- Regulation of expression of human RNA polymerase II-transcribed snRNA genes.Open biology · 2017Review
- The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression.The EMBO journal · 2017Article
- Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.Nucleic acids research · 2016Article
- Overview of CDK9 as a target in cancer research.Cell cycle (Georgetown, Tex.) · 2016Review
- Integrator: surprisingly diverse functions in gene expression.Trends in biochemical sciences · 2015Review
- CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes.Nature structural & molecular biology · 2015Article
- MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.Nature communications · 2015Article
- CTCF regulates NELF, DSIF and P-TEFb recruitment during transcription.Transcription · 2015Article
- A kinase-independent activity of Cdk9 modulates glucocorticoid receptor-mediated gene induction.Biochemistry · 2014Article
- Article
- Human snRNA genes use polyadenylation factors to promote efficient transcription termination.Nucleic acids research · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Phosphorylation of Ser2 of the heptapeptide repeat of the CTD of mammalian pol II by P-TEFb is associated with productive elongation of transcription of protein-coding genes. Here, we show that the CTD of pol II transcribing the human U2 snRNA genes is phosphorylated on Ser2 in vivo and that both the CDK9 kinase and cyclin T components of P-TEFb are required for cotranscriptional recognition of the 3' box RNA 3' end processing signal. However, inhibitors of CDK9 do not affect transcription of the U2 genes, indicating that P-TEFb functions exclusively as an RNA processing factor in expression of these relatively short, intronless genes. We also show that inhibition of CDK9 does not adversely affect either transcription of an intron-less, replication-activated histone H2b gene or recognition of the histone gene-specific U7-dependent RNA 3' end formation signal. These results emphasize that the role of P-TEFb as an activator of transcription elongation can be separated from its role in RNA processing and that neither function is universally required for expression of mammalian pol II-dependent genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.