ArticlePlant molecular biology2005
Laser capture microdissection (LCM) and expression analyses of Glycine max (soybean) syncytium containing root regions formed by the plant pathogen Heterodera glycines (soybean cyst nematode).
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 125 citations in OpenAlex.
- RNA Isolation from Nematode-Induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Putative Plasma Membrane NaFrontiers in plant science · 2022Article
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- Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots.International journal of molecular sciences · 2021Article
- A Bacillus thuringiensis Cry protein controls soybean cyst nematode in transgenic soybean plants.Nature communications · 2021Article
- Article
- An optimized protocol for isolation of high-quality RNA through laser capture microdissection of leaf material.Plant direct · 2019Article
- Transcriptome profiling of Cucumis metuliferus infected by Meloidogyne incognita provides new insights into putative defense regulatory network in Cucurbitaceae.Scientific reports · 2017Article
- An Efficient LCM-Based Method for Tissue Specific Expression Analysis of Genes and miRNAs.Scientific reports · 2016Article
- An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection.Plant methods · 2016Article
- Decipher the Molecular Response of Plant Single Cell Types to Environmental Stresses.BioMed research international · 2016Review
- Comparative Transcriptome Analysis of Resistant and Susceptible Common Bean Genotypes in Response to Soybean Cyst Nematode Infection.PloS one · 2016Article
- Spatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague.mBio · 2015Article
- Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.Molecular biotechnology · 2015Review
- Syntaxin 31 functions in Glycine max resistance to the plant parasitic nematode Heterodera glycines.Plant molecular biology · 2014Article
- Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots.BMC plant biology · 2014Article
- Article
- Unleashing the potential of the root hair cell as a single plant cell type model in root systems biology.Frontiers in plant science · 2013Article
- The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.Plant molecular biology · 2012Article
- Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica.Genetics and molecular biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Roots of soybean, Glycine max cv. Kent L. Merr., plants susceptible to the soybean cyst nematode (SCN), Heterodera glycines Ichinohe, were inoculated and allowed to develop feeding sites (syncytia) for 8 days. Root samples enriched in syncytial cells were collected using laser capture microdissection (LCM). RNA was extracted and used to make a cDNA library and expressed sequence tags (ESTs) were produced and used for a Gene Ontology (GO) analysis. RT-PCR results indicated enhanced expression of an aquaporin (GmPIP2,2), alpha-tubulin (GmTubA1), beta-tubulin (GmTubB4) and several other genes in syncytium-enriched samples as compared to samples extracted from whole roots. While RT-PCR data showed increased transcript levels of GmPIP2,2 from LCM tissue enriched in syncytial cells, in situ hybridization showed prominent GmPIP2,2 hybridization to RNA in the parenchymal cells tightly juxtaposed to the syncytium. Immunolocalization indicated stronger alpha-tubulin signal within the syncytium as compared to surrounding tissue. However, alpha-tubulin labeling appeared diffuse or clumped. Thus, LCM allowed for the isolation of tissue enriched for syncytial cells, providing material suitable for a variety of molecular analyses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.