ArticlePlant molecular biology2005
The maize An2 gene is induced by Fusarium attack and encodes an ent-copalyl diphosphate synthase.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 133 citations in OpenAlex.
- Discovery of the rosalexin pathway expands the modular network of maize diterpenoid phytoalexins.Research square · 2026Article
- Characterization of the entry steps in diterpenoid alkaloid biosynthesis.Molecular plant · 2026Article
- A Review of Plant-Derived Diterpenoid Biosynthesis: From Structural Scaffold Diversity and Lineage-Associated Distribution to Enzyme Mining and Discovery Strategies.Molecules (Basel, Switzerland) · 2026Review
- Genome-wide discovery and characterization of terpene synthases contributing to strawberry aroma metabolism.Plant physiology · 2026Article
- qPHThe plant genome · 2025Article
- Copalyl diphosphate synthase gene TaCPS1 mediated resistance to Fusarium graminearum in wheat in response to Piriformospora indica colonization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Entry Steps in the Biosynthetic Pathway to Diterpenoid Alkaloids inbioRxiv : the preprint server for biology · 2025Article
- Genetic Diversity Analysis and GWAS of Plant Height and Ear Height in Maize Inbred Lines from South-East China.Plants (Basel, Switzerland) · 2025Article
- Phenotypic characterization and genetic mapping of the semi-dwarf mutant sdw9 in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Genome-wide association study and molecular marker development for susceptibility to Gibberella ear rot in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Sequence-Structure Analysis Unlocking the Potential Functional Application of the Local 3D Motifs of Plant-Derived Diterpene Synthases.Biomolecules · 2024Article
- The terpene synthase gene family in maize - a clarification of existing community nomenclature.BMC genomics · 2023Article
- Genome-wide identification and expression analysis of terpene synthases in Gossypium species in response to gossypol biosynthesis.Functional & integrative genomics · 2023Article
- A dolabralexin-deficient mutant provides insight into specialized diterpenoid metabolism in maize.Plant physiology · 2023Article
- Maize Terpene Synthase 8 (ZmTPS8) Contributes to a Complex Blend of Fungal-Elicited Antibiotics.Plants (Basel, Switzerland) · 2023Article
- Specialized metabolites as mediators for plant-fungus crosstalk and their evolving roles.Current opinion in plant biology · 2021Review
- Cytochrome P450-catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.).The Plant journal : for cell and molecular biology · 2021Article
- Functional identification of the terpene synthase family involved in diterpenoid alkaloids biosynthesis inActa pharmaceutica Sinica. B · 2021Article
- A maize leucine-rich repeat receptor-like protein kinase mediates responses to fungal attack.Planta · 2021Article
- Genome-wide detection and classification of terpene synthase genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
10 more citing papers are in PubMed but not listed here.
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9 authors at 2 institutions in 2 countries.
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Abstract
Using the technique of differential display, a maize transcript was identified whose silk tissue expression is induced in the presence of the ear rot pathogen Fusarium graminearum. The 3445 nt transcript includes a 727 nt 5' untranslated leader with the potential for extensive secondary structure and represents the maize gene An2. An2 encodes a copalyl diphosphate synthase (CPS)-like protein with 60% amino acid sequence identity with the maize An1 gene product involved in gibberellin (GA) biosynthesis. Recombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent-copalyl diphosphate (ent-CPP) synthases (ent-CPS). Notably, the presence of an additional ent-CPS gene is consistent with previous reports that maize GA biosynthesis can proceed in the absence of An1. In addition, northern blot analysis showed that An2 transcript levels were strongly up-regulated by Fusarium attack, with an increase in silk, husk and ear tip tissues as early as 6 h after inoculation of silk channels with spore suspensions of various Fusarium sp. Gene expression of a third maize CPS-like gene, Cpsl1, is not affected by Fusarium infection. The Fusarium-inducible nature of An2 is also consistent with a previous report that cell-free extracts from maize seedlings produce ent-CPP derived diterpenes in response to Fusarium infection. However, it is not known whether An2 is involved in defense-related secondary metabolism in addition to GA synthesis.
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