ArticleJournal of virology2005
A point mutation within the replicase gene differentially affects coronavirus genome versus minigenome replication.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Review
- Review
- Identification of intrinsically disorder regions in non-structural proteins of SARS-CoV-2: New insights into drug and vaccine resistance.Molecular and cellular biochemistry · 2022Article
- Article
- Structure of Nonstructural Protein 1 from SARS-CoV-2.Journal of virology · 2021Article
- Structure of nonstructural protein 1 from SARS-CoV-2.bioRxiv : the preprint server for biology · 2020Article
- Coronavirus nonstructural protein 1: Common and distinct functions in the regulation of host and viral gene expression.Virus research · 2015Review
- Article
- Atlas of coronavirus replicase structure.Virus research · 2014Review
- Structure of alphacoronavirus transmissible gastroenteritis virus nsp1 has implications for coronavirus nsp1 function and evolution.Journal of virology · 2013Article
- Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation.Virus genes · 2010Article
- Host cell proteins interacting with the 3' end of TGEV coronavirus genome influence virus replication.Virology · 2009Article
- Novel beta-barrel fold in the nuclear magnetic resonance structure of the replicase nonstructural protein 1 from the severe acute respiratory syndrome coronavirus.Journal of virology · 2007Article
- Human coronavirus 229E papain-like proteases have overlapping specificities but distinct functions in viral replication.Journal of virology · 2007Article
- Helper virus-independent trans-replication of hepatitis C virus-derived minigenome.Biochemical and biophysical research communications · 2007Article
- Biochemical aspects of coronavirus replication.Advances in experimental medicine and biology · 2006Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the construction of the transmissible gastroenteritis virus (TGEV) full-length cDNA clone, a point mutation at position 637 that was present in the defective minigenome DI-C was maintained as a genetic marker. Sequence analysis of the recovered viruses showed a reversion at this position to the original virus sequence. The effect of point mutations at nucleotide 637 was analyzed by reverse genetics using a TGEV full-length cDNA clone and cDNAs from TGEV-derived minigenomes. The replacement of nucleotide 637 of TGEV genome by a T, as in the DI-C sequence, or an A severely affected virus recovery from the cDNA, yielding mutant viruses with low titers and small plaques compared to those of the wild type. In contrast, T or A at position 637 was required for minigenome rescue in trans by the helper virus. No relationship between these observations and RNA secondary-structure predictions was found, indicating that mutations at nucleotide 637 most likely had an effect at the protein level. Nucleotide 637 occupies the second codon position at amino acid 108 of the pp1a polyprotein. This position is predicted to map in the N-terminal polyprotein papain-like proteinase (PLP-1) cleavage site at the p9/p87 junction. Replacement of G-637 by A, which causes a drastic amino acid change (Gly to Asp) at position 108, affected PLP-1-mediated cleavage in vitro. A correlation was found between predicted cleaving and noncleaving mutations and efficient virus rescue from cDNA and minigenome amplification, respectively.
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