ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Phorbol esters target the activity-dependent recycling pool and spare spontaneous vesicle recycling.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
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- Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.BMC biology · 2021Article
- Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.International journal of molecular sciences · 2019Article
- Presynaptic origins of distinct modes of neurotransmitter release.Current opinion in neurobiology · 2018Review
- Calcium dependence of spontaneous neurotransmitter release.Journal of neuroscience research · 2018Review
- The antidepressant fluoxetine mobilizes vesicles to the recycling pool of rat hippocampal synapses during high activity.Molecular neurobiology · 2014Article
- Reelin mobilizes a VAMP7-dependent synaptic vesicle pool and selectively augments spontaneous neurotransmission.Neuron · 2013Article
- Vti1a identifies a vesicle pool that preferentially recycles at rest and maintains spontaneous neurotransmission.Neuron · 2012Article
- The role of non-canonical SNAREs in synaptic vesicle recycling.Cellular logistics · 2012Article
- Calcium-dependent isoforms of protein kinase C mediate posttetanic potentiation at the calyx of Held.Neuron · 2011Article
- Use-dependent AMPA receptor block reveals segregation of spontaneous and evoked glutamatergic neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Differential regulation of spontaneous and evoked neurotransmitter release at central synapses.Current opinion in neurobiology · 2011Review
- Spontaneous glutamate release is independent of calcium influx and tonically activated by the calcium-sensing receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Gender-Specific Mechanism of Synaptic Impairment and Its Prevention by GCSF in a Mouse Model of ALS.Frontiers in cellular neuroscience · 2011Article
- Postsynaptic Neuroligin1 regulates presynaptic maturation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- beta-Phorbol ester-induced enhancement of exocytosis in large mossy fiber boutons of mouse hippocampus.The journal of physiological sciences : JPS · 2009Article
- Development regulates a switch between post- and presynaptic strengthening in response to activity deprivation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Spontaneous and evoked glutamate release activates two populations of NMDA receptors with limited overlap.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Elementary properties of spontaneous fusion of peptidergic vesicles: fusion pore gating.The Journal of physiology · 2007Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Using electrophysiology and styryl dye imaging, we studied the effect of phorbol 12-myristate 13-acetate (PMA) on activity-dependent and spontaneous vesicle recycling. In electrophysiological experiments, we found that the PMA effect depended on the maturational state of the synapses. Spontaneous neurotransmitter release from nascent synapses without a functional readily releasable pool (RRP) was unresponsive to PMA application. In contrast, mature synapses responded robustly to PMA application, consistent with previous studies. Using styryl dye imaging, we found that there was a PMA-dependent increase in the size of the RRP when PMA was present before, during, or after activity-dependent dye uptake, suggesting that this effect involves an increase in the population of the RRP by vesicles recruited from the reserve pool. Additionally, we found that when PMA was present during spontaneous dye uptake, there was an increase in dye labeling, and these additional dye-loaded vesicles showed rapid destaining in response to strong stimulation and were also releasable by hypertonic sucrose. In contrast, these observations were not reproducible when PMA treatment was performed after spontaneous dye uptake and extracellular dye washout. Together, these findings suggest that the increased spontaneous neurotransmission in the presence of PMA was attributable to release of vesicles from the RRP rather than an effect of PMA on the spontaneously recycling pool. Thus, the phorbol esters selectively regulate the activity-dependent pool of vesicles, indicating that priming mechanisms that prepare vesicles for fusion, which are targeted by phorbol esters, are different for the spontaneous and evoked forms of fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.