ArticleNucleic acids research2005
In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Gene Editing in Hematopoietic Stem Cells.Advances in experimental medicine and biology · 2023Review
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.Nucleic acids research · 2017Article
- In Vivo Delivery Systems for Therapeutic Genome Editing.International journal of molecular sciences · 2016Review
- Article
- Reprogramming homing endonuclease specificity through computational design and directed evolution.Nucleic acids research · 2014Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Germline excision of transgenes in Aedes aegypti by homing endonucleases.Scientific reports · 2013Article
- Improved modeling of side-chain--base interactions and plasticity in protein--DNA interface design.Journal of molecular biology · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Precision editing of large animal genomes.Advances in genetics · 2012Review
- Native homing endonucleases can target conserved genes in humans and in animal models.Nucleic acids research · 2011Article
- Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.Structure (London, England : 1993) · 2011Review
- Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.Journal of molecular biology · 2011Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display.Nucleic acids research · 2009Article
- Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.Insect molecular biology · 2009Article
- Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity.Nucleic acids research · 2009Article
- Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.Genome research · 2009Article
- Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to engineer novel endonucleases with tailored specificities is under strong investigation. In this report, we present a simple and efficient method to select meganucleases from libraries of variants, based on their cleavage properties. The method has the advantage of directly selecting for the ability to induce double-strand break induced homologous recombination in a eukaryotic environment. Model selections demonstrated high levels of enrichments. Moreover, this method compared favorably with phage display for enrichment of active mutants from a mutant library. This approach makes possible the exploration of large sequence spaces and thereby represents a valuable tool for genome engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.