ArticlePlant physiology2005
Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 217 citations in OpenAlex.
- Metabolomic Profiling ofJournal of fungi (Basel, Switzerland) · 2026Article
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds.Communications engineering · 2025Article
- Metabolic Niches and Plasticity of Sand-Dune Plant Communities Along a Trans-European Gradient.Metabolites · 2025Article
- Intracellular metabolomic profiling of Picochlorum sp. under diurnal conditions mimicking outdoor light, temperature, and seasonal variations.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Metabolomic response to high light from pgrl1 and pgr5 mutants of Chlamydomonas reinhardtii.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Interactions Between Carbon Metabolism and Photosynthetic Electron Transport in aFrontiers in plant science · 2022Article
- Global Metabolomics Reveals ThatFrontiers in bioengineering and biotechnology · 2021Article
- Plant Single-Cell Metabolomics-Challenges and Perspectives.International journal of molecular sciences · 2020Review
- Mutations in PIH proteins MOT48, TWI1 and PF13 define common and unique steps for preassembly of each, different ciliary dynein.PLoS genetics · 2020Article
- Roles of metabolic regulation in developing Quercus variabilis acorns at contrasting geologically-derived phosphorus sites in subtropical China.BMC plant biology · 2020Article
- In-depth Understanding ofInternational journal of molecular sciences · 2020Article
- Short-Term Temporal Metabolic Behavior in Halophilic CyanobacteriumMetabolites · 2019Article
- The influence of four pharmaceuticals on Chlorellapyrenoidosa culture.Scientific reports · 2019Article
- Nitrogen-dependent coordination of cell cycle, quiescence and TAG accumulation in Chlamydomonas.Biotechnology for biofuels · 2019Review
- Time-resolved carotenoid profiling and transcriptomic analysis reveal mechanism of carotenogenesis for astaxanthin synthesis in the oleaginous green algaBiotechnology for biofuels · 2019Article
- Quenching for Microalgal Metabolomics: A Case Study on the Unicellular Eukaryotic Green AlgaMetabolites · 2018Article
- Recovery from N Deprivation Is a Transcriptionally and Functionally Distinct State inPlant physiology · 2018Article
- Growth and lipid accumulation by different nutrients in the microalgaBiotechnology for biofuels · 2018Article
- Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols.Metabolites · 2017Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.