ArticleMolecular and cellular biology1992
Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- Promoters, KeyInternational journal of molecular sciences · 2024Review
- Review
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- Identification of defense-related genes in rice responding to challenge by Rhizoctonia solani.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Cytokinins in the bryophyte Physcomitrella patens: analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins.Plant physiology · 2007Article
- Isolation and characterization of novel defense response genes involved in compatible and incompatible interactions between rice and Magnaporthe grisea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation.Plant molecular biology · 1995Article
- Article
- Abundance, polymorphism and genetic mapping of microsatellites in rice.Molecular & general genetics : MGG · 1993Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The promoter of the constitutively expressed rice (Oryza sativa) actin 1 gene (Act1) is highly active in transformed rice plants (W. Zhang, D. McElroy, and R. Wu, Plant Cell 3:1150-1160, 1991). A region 834 bp upstream of the Act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (D. McElroy, W. Zhang, J. Cao, and R. Wu, Plant Cell 2:163-171, 1990). We have constructed a series of Act1 promoter deletions fused to a bacterial beta-glucuronidase reporter sequence (Gus). Transient expression assays in transformed rice protoplasts, as well as transformed maize cells and tissues, identified two distinct cis-acting regulatory elements in the Act1 promoter. A 38-bp poly(dA-dT) region was found to be a positive regulator of Act1 promoter activity. Deletion of the poly(dA-dT) element lowered Gus expression by at least threefold compared with expression produced by the full-length Act1 promoter. By gel retardation and footprinting, we identified a ubiquitous rice protein which specifically recognizes this poly(dA-dT) element in the constitutively active Act1 promoter. A CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act1 promoter in transformed rice protoplasts. Transient expression assays in different maize cells and tissues with use of the Act1 deletion constructs suggested that the CCCAA pentamer repeat region functions in a complex tissue-specific manner. A CCCAA-binding protein was detected only in root extracts.
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