ArticleInvestigative ophthalmology & visual science2005
Cone survival despite rod degeneration in XOPS-mCFP transgenic zebrafish.
Article in Investigative ophthalmology & visual science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 111 citations in OpenAlex.
- Microglia response and function in a chronic model of photoreceptor damage.Frontiers in cell and developmental biology · 2025Article
- Review
- Ancient origin of the rod bipolar cell pathway in the vertebrate retina.Nature ecology & evolution · 2024Article
- Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina.Development (Cambridge, England) · 2024Article
- Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration.eLife · 2023Article
- Molecular basis of retinal remodeling in a zebrafish model of retinitis pigmentosa.Cellular and molecular life sciences : CMLS · 2023Article
- Losing, preserving, and restoring vision from neurodegeneration in the eye.Current biology : CB · 2023Review
- Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina.bioRxiv : the preprint server for biology · 2023Article
- Chromatin remodeler Chd7 regulates photoreceptor development and outer segment length.Experimental eye research · 2023Article
- Eye flukes (Diplostomum spp) damage retinal tissue and may cause a regenerative response in wild threespine stickleback fish.Experimental eye research · 2022Article
- Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors.Journal of cell science · 2022Article
- Article
- Notch Inhibition Promotes Regeneration and Immunosuppression Supports Cone Survival in a Zebrafish Model of Inherited Retinal Dystrophy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- NTR 2.0: a rationally engineered prodrug-converting enzyme with substantially enhanced efficacy for targeted cell ablation.Nature methods · 2022Article
- Zebrafish models of inherited retinal dystrophies.Journal of translational genetics and genomics · 2022Article
- Development and characterization of a chronic photoreceptor degeneration model in adult zebrafish that does not trigger a regenerative response.Experimental eye research · 2021Article
- Metabolism in the Zebrafish Retina.Journal of developmental biology · 2021Review
- Zebrafish Models of Photoreceptor Dysfunction and Degeneration.Biomolecules · 2021Review
- Loss of Pde6a Induces Rod Outer Segment Shrinkage and Visual Alterations inFrontiers in cell and developmental biology · 2021Article
- Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeIn animal models of retinitis pigmentosa, rod photoreceptor degeneration eventually leads to loss of cone photoreceptors. The purpose of this study was to characterize a transgenic model of rod degeneration in zebrafish.
methodsZebrafish transgenic for XOPS-mCFP, a membrane-targeted form of cyan fluorescent protein driven by the Xenopus rhodopsin promoter, were generated by plasmid injection. Immunohistochemistry was used to detect cell type, proliferation, and TUNEL markers in larval and adult retinas. Rod- and cone-specific transcripts were detected by RT-PCR. Visual responses in transgenic adults were measured by electroretinogram.
resultsThe XOPS promoter directed specific expression of mCFP in rods by 55 hours post fertilization (hpf). Rods in XOPS-mCFP heterozygotes began dying at 3.5 days post fertilization (dpf) and were almost completely absent by 5 dpf. A few rods were observed at the retinal margin, and numerous immature rods were observed in the outer nuclear layer (ONL) of transgenic adults. Apoptosis was increased in the ONL of larval and adult transgenic animals, and an elevation of rod precursor proliferation in adults was observed. ERG analysis confirmed that rod responses were absent in this line. Cone morphology and electrophysiology appeared normal in transgenic animals up to 7 months of age.
conclusionsThe XOPS-mCFP transgene causes selective degeneration of rods without secondary loss of cones in animals up to 7 months of age. This raises important questions about the significance of rod-cone interactions in zebrafish and their potential as a model of human inherited retinal degenerations.
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