ArticleNature methods2005
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
Article in Nature methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 117 citations in OpenAlex.
- Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase.Genetics · 2026Article
- There and turn back again: the application of phage serine integrases in eukaryotic systems.Frontiers in bioengineering and biotechnology · 2025Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- The Making of TransgenicMethods and protocols · 2020Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Review
- Modeling Dominant and Recessive Forms of Retinitis Pigmentosa by Editing Three Rhodopsin-Encoding Genes in Xenopus Laevis Using Crispr/Cas9.Scientific reports · 2017Article
- Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.Cellular and molecular life sciences : CMLS · 2015Review
- Genome editing for crop improvement: Challenges and opportunities.GM crops & food · 2015Article
- Hybrid lentivirus-phiC31-int-NLS vector allows site-specific recombination in murine and human cells but induces DNA damage.PloS one · 2014Article
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Dual promoter expression system with insulator ensures a stringent tissue-specific regulation of two reporter genes in the transgenic fish.Transgenic research · 2013Article
- Cartilage on the move: cartilage lineage tracing during tadpole metamorphosis.Development, growth & differentiation · 2012Article
- Optimized transgenesis in Xenopus laevis/gilli isogenetic clones for immunological studies.Genesis (New York, N.Y. : 2000) · 2012Article
- Article
- Using ΦC31 integrase to mediate insertion of DNA in Xenopus embryos.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Genetic analysis of Xenopus tropicalis.Methods in molecular biology (Clifton, N.J.) · 2012Article
- pTransgenesis: a cross-species, modular transgenesis resource.Development (Cambridge, England) · 2011Article
- New doxycycline-inducible transgenic lines in Xenopus.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacteriophage phiC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA. Here we show that the coinjection of mRNA encoding phiC31 integrase with plasmid DNA encoding the green fluorescent protein (GFP) can be used to generate transgenic X. laevis embryos. Despite integration into the genome, appropriate promoter expression required modification of the reporter plasmid by bracketing the GFP reporter gene with tandem copies of the chicken beta-globin 5' HS4 insulator to relieve silencing owing to chromatin position effects. These experiments demonstrate that the integration of insulated gene sequences using phiC31 integrase can be used to efficiently create transgenic embryos in X. laevis and may increase the practical use of phiC31 integrase in other systems as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.