ArticleInfection and immunity2005
Identification of novel antigenic proteins in a complex Anaplasma marginale outer membrane immunogen by mass spectrometry and genomic mapping.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 105 citations in OpenAlex.
- Haematology, serum biochemistry, innate and adaptive immune system responses to blood pathogen infections in cattle: A review.Parasitology research · 2026Review
- Molecular Identification and Bioinformatics Analysis ofPathogens (Basel, Switzerland) · 2024Article
- Identification ofInfection and immunity · 2024Article
- Article
- Detection and Characterisation of Anaplasma marginale and A. centrale in South Africa.Veterinary sciences · 2018Review
- A hybrid protein containing MSP1a repeats and Omp7, Omp8 and Omp9 epitopes protect immunized BALB/c mice against anaplasmosis.Veterinary research · 2018Article
- Identification of a T-Cell Epitope That Is Globally Conserved among Outer Membrane Proteins (OMPs) OMP7, OMP8, and OMP9 of Anaplasma marginale Strains and with OMP7 from the A. marginale subsp. centrale Vaccine Strain.Clinical and vaccine immunology : CVI · 2017Article
- Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System.Nanomaterials (Basel, Switzerland) · 2016Article
- Anaplasma marginale Actively Modulates Vacuolar Maturation during Intracellular Infection of Its Tick Vector, Dermacentor andersoni.Applied and environmental microbiology · 2016Article
- Immunogenicity of Outer Membrane Proteins VirB9-1 and VirB9-2, a Novel Nanovaccine against Anaplasma marginale.PloS one · 2016Article
- Anaplasma marginale: Diversity, Virulence, and Vaccine Landscape through a Genomics Approach.BioMed research international · 2016Review
- Immunoproteomic profiling of Rickettsia parkeri and Rickettsia amblyommii.Ticks and tick-borne diseases · 2015Article
- Reduced Infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale.Applied and environmental microbiology · 2015Article
- Article
- Immunoproteomic analysis to identify Shiga toxin-producing Escherichia coli outer membrane proteins expressed during human infection.Infection and immunity · 2014Article
- Immunization-induced Anaplasma marginale-specific T-lymphocyte responses impaired by A. marginale infection are restored after eliminating infection with tetracycline.Clinical and vaccine immunology : CVI · 2014Article
- Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis.BMC genomics · 2014Article
- Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale.Current microbiology · 2014Article
- Linkage between Anaplasma marginale outer membrane proteins enhances immunogenicity but is not required for protection from challenge.Clinical and vaccine immunology : CVI · 2013Article
- Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Immunization with purified Anaplasma marginale outer membranes induces complete protection against infection that is associated with CD4+ T-lymphocyte-mediated gamma interferon secretion and immunoglobulin G2 (IgG2) antibody titers. However, knowledge of the composition of the outer membrane immunogen is limited. Recent sequencing and annotation of the A. marginale genome predicts at least 62 outer membrane proteins (OMP), enabling a proteomic and genomic approach for identification of novel OMP by use of IgG serum antibody from outer membrane vaccinates. Outer membrane proteins were separated by two-dimensional electrophoresis, and proteins recognized by total IgG and IgG2 in immune sera of outer membrane-vaccinated cattle were detected by immunoblotting. Immunoreactive protein spots were excised and subjected to liquid chromatography-tandem mass spectrometry. A database search of the A. marginale genome identified 24 antigenic proteins that were predicted to be outer membrane, inner membrane, or membrane-associated proteins. These included the previously characterized surface-exposed outer membrane proteins MSP2, operon associated gene 2 (OpAG2), MSP3, and MSP5 as well as recently identified appendage-associated proteins. Among the 21 newly described antigenic proteins, 14 are annotated in the A. marginale genome and include type IV secretion system proteins, elongation factor Tu, and members of the MSP2 superfamily. The identification of these novel antigenic proteins markedly expands current understanding of the composition of the protective immunogen and provides new candidates for vaccine development.
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