ArticleInfection and immunity2005
Virulence and karyotype analyses of rad52 mutants of Candida albicans: regeneration of a truncated chromosome of a reintegrant strain (rad52/RAD52) in the host.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Mec1-Rad53 Signaling Regulates DNA Damage-Induced Autophagy and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2023Article
- The δ subunit of FNature communications · 2021Article
- Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.Current genetics · 2021Review
- DNA damage checkpoint and repair: From the budding yeastComputational and structural biotechnology journal · 2021Review
- Wss1 homolog from Candida albicans and its role in DNA-protein crosslink tolerance.Molecular microbiology · 2020Article
- Loss of Arp1, a putative actin-related protein, triggers filamentous and invasive growth and impairs pathogenicity inComputational and structural biotechnology journal · 2020Article
- The Influence of Genetic Stability onG3 (Bethesda, Md.) · 2018Article
- Mitochondrial Complex V α Subunit Is Critical forFrontiers in microbiology · 2017Article
- The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans.FEMS yeast research · 2015Article
- The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways.PLoS pathogens · 2015Article
- Chromatin-mediated Candida albicans virulence.Biochimica et biophysica acta · 2012Article
- Candida albicans SRR1, a putative two-component response regulator gene, is required for stress adaptation, morphogenesis, and virulence.Eukaryotic cell · 2011Article
- Rad52 function prevents chromosome loss and truncation in Candida albicans.Molecular microbiology · 2011Article
- Histone acetyltransferase Rtt109 is required for Candida albicans pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage responses, morphogenesis, and virulence.Eukaryotic cell · 2009Article
- Genotypic evolution of azole resistance mechanisms in sequential Candida albicans isolates.Eukaryotic cell · 2007Article
- Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-DeltaDelta strains of Candida albicans to sorbose.Fungal genetics and biology : FG & B · 2007Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The virulence of Candida albicans mutants lacking one or both copies of RAD52, a gene involved in homologous recombination (HR), was evaluated in a murine model of hematogenously disseminated candidiasis. In this study, the virulence of the rad52Delta mutant was dependent upon the inoculum concentration. Mice survived at a cell inoculum of 1 x 10(6), but there was a decrease in survival time at dosages of 1.5 x 10(6) and especially at 3 x 10(6) cells per animal. The heterozygote RAD52/rad52 behaved like wild type, whereas a reintegrant strain was intermediate in its ability to cause death compared to these strains and to the avirulent rad52/rad52 null at inocula of 1 x 10(6) and 1.5 x 10(6) cells. A double mutant, lig4/lig4/rad52/rad52, was avirulent at all inocula used. PCR analysis of the RAD52 and/or LIG4 loci showed that all strains recovered from animals matched the genotype of the inoculated strains. Analysis of the electrophoretical karyotypes indicated that the inoculated, reintegrant strain carried a large deletion in one copy of chromosome 6 (the shortest homologue, or Chr6b). Interestingly, truncated Chr6b was regenerated in all the strains recovered from moribund animals using the homologue as a template. Further, regeneration of Chr6b was paralleled by an increase in virulence that was still lower than that of wild type, likely because of the persistent loss of heterozygosity in the regenerated region. Overall, our results indicate that systemic candidiasis can develop in the absence of HR, but simultaneous elimination of both recombination pathways, HR and nonhomologous end-joining, suppresses virulence even at very high inocula.
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