ReviewExpert opinion on drug delivery2004
Intracellular trafficking of nucleic acids.
Review in Expert opinion on drug delivery, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Stimulus-Responsive Polymeric Carriers for Gene Delivery: Balancing Endosomal Escape with Nucleic Acid Release.Pharmaceutical research · 2026Review
- Review
- Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity.Cell reports. Medicine · 2025Article
- Optogenetics: Illuminating the Future of Hearing Restoration and Understanding Auditory Perception.Current gene therapy · 2024Review
- Biologics and their delivery systems: Trends in myocardial infarction.Advanced drug delivery reviews · 2021Review
- BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Miniaturization of gene transfection assays in 384- and 1536-well microplates.Analytical biochemistry · 2015Article
- Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules.Gene therapy · 2013Article
- Performance of high quality minicircle DNA for in vitro and in vivo gene transfer.Molecular biotechnology · 2013Article
- Barriers to non-viral vector-mediated gene delivery in the nervous system.Pharmaceutical research · 2011Review
- Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics.Biotechnology and bioengineering · 2011Article
- Co-localization of the amyloid precursor protein and Notch intracellular domains in nuclear transcription factories.Neurobiology of aging · 2010Article
- A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine.Journal of virology · 2009Article
- Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer.Gene therapy · 2006Article
- Nonviral gene transfer to skeletal, smooth, and cardiac muscle in living animals.American journal of physiology. Cell physiology · 2005Review
- A novel pathway to detect and cope with exogenous dsDNA.Communicative & integrative biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Until recently, the attention of most researchers has focused on the first and last steps of gene transfer, namely delivery to the cell and transcription, in order to optimise transfection and gene therapy. However, over the past few years, researchers have realised that the intracellular trafficking of plasmids is more than just a "black box" and is actually one of the major barriers to effective gene delivery. After entering the cytoplasm, following direct delivery or endocytosis, plasmids or other vectors must travel relatively long distances through the mesh of cytoskeletal networks before reaching the nuclear envelope. Once at the nuclear envelope, the DNA must either wait until cell division, or be specifically transported through the nuclear pore complex, in order to reach the nucleoplasm where it can be transcribed. This review focuses on recent developments in the understanding of these intracellular trafficking events as they relate to gene delivery. Hopefully, by continuing to unravel the mechanisms by which plasmids and other gene delivery vectors move throughout the cell, and by understanding the cell biology of gene transfer, superior methods of transfection and gene therapy can be developed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.