ArticleThe European physical journal. E, Soft matter2005
Detection of submicron-sized raft-like domains in membranes by small-angle neutron scattering.
Article in The European physical journal. E, Soft matter, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Temperature dependence of membrane viscosity of ternary lipid GUV with LBiophysical journal · 2025Article
- Cholesterol and Lipid Rafts in the Biogenesis of Amyloid-β Protein and Alzheimer's Disease.Annual review of biophysics · 2024Review
- Small-angle scattering from flat bilayers containing correlated scattering length density inhomogeneities.Journal of applied crystallography · 2023Article
- Biophysical studies of lipid nanodomains using different physical characterization techniques.Biophysical journal · 2023Review
- Article
- Influence of ceramide on lipid domain stability studied with small-angle neutron scattering: The role of acyl chain length and unsaturation.Chemistry and physics of lipids · 2022Article
- Improved chemical and isotopic labeling of biomembranes inFrontiers in molecular biosciences · 2022Article
- Accumulation of styrene oligomers alters lipid membrane phase order and miscibility.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biomembrane Structure and Material Properties Studied With Neutron Scattering.Frontiers in chemistry · 2021Review
- Laterally Resolved Small-Angle Scattering Intensity from Lipid Bilayer Simulations: An Exact and a Limited-Range Treatment.Journal of chemical theory and computation · 2020Article
- Creating Asymmetric Phospholipid Vesicles via Exchange With Lipid-Coated Silica Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Interleaflet Coupling of Lipid Nanodomains - Insights FromFrontiers in cell and developmental biology · 2020Review
- Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.Biophysical journal · 2018Article
- The actions of volatile anesthetics: a new perspective.Acta crystallographica. Section D, Structural biology · 2018Review
- Complex biomembrane mimetics on the sub-nanometer scale.Biophysical reviews · 2017Review
- Article
- On scattered waves and lipid domains: detecting membrane rafts with X-rays and neutrons.Soft matter · 2015Article
- Structural Significance of Lipid Diversity as Studied by Small Angle Neutron and X-ray Scattering.Membranes · 2015Review
- Phase behavior and domain size in sphingomyelin-containing lipid bilayers.Biochimica et biophysica acta · 2013Article
- Structure determination of functional membrane proteins using small-angle neutron scattering (sans) with small, mixed-lipid liposomes: native beef heart mitochondrial cytochrome c oxidase forms dimers.The protein journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Using coarse grained models of heterogeneous vesicles we demonstrate the potential for small-angle neutron scattering (SANS) to detect and distinguish between two different categories of lateral segregation: 1) unilamellar vesicles (ULV) containing a single domain and 2) the formation of several small domains or "clusters" (approximately 10 nm in radius) on a ULV. Exploiting the unique sensitivity of neutron scattering to differences between hydrogen and deuterium, we show that the liquid ordered (lo) DPPC-rich phase can be selectively labeled using chain deuterated dipalymitoyl phosphatidylcholine (dDPPC), which greatly facilitates the use of SANS to detect membrane domains. SANS experiments are then performed in order to detect and characterize, on nanometer length scales, lateral heterogeneities, or so-called "rafts", in approximately 30 nm radius low polydispersity ULV made up of ternary mixtures of phospholipids and cholesterol. For 1:1:1 DOPC:DPPC:cholesterol (DDC) ULV we find evidence for the formation of lateral heterogeneities on cooling below 30 degrees C. These heterogeneities do not appear when DOPC is replaced by SOPC. Fits to the experimental data using coarse grained models show that, at room temperature, DDC ULV each exhibit approximately 30 domains with average radii of approximately 10 nm.
Indexed as
Identifiers
16292472What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.